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Cloning and characterization of HIV-1-inducible astrocyte elevated gene-1, AEG-1
Kang DC, Su ZZ, Sarkar D, Emdad L, Volsky DJ, Fisher PB.. Gene, 2005-06-01
Topic: Brain proteins, microglia and lab models
We presently describe the full-length cloning and functional characterization of an HIV-1-inducible gene, astrocyte elevated gene (AEG)-1. Additionally, a novel method is outlined for producing tag-free recombinant protein in a baculovirus system and its use in producing AEG-1 protein. AEG-1 mRNA is expressed ubiquitously with higher expression in tissues containing muscular actin and its expression is increased in astrocytes infected with HIV-1 or treated with gp120 or tumor necrosis factor (TNF)-alpha.
Cite this study APA, Vancouver, BibTeX · download .bib or .ris
APA 7th
Kang, D. C., Su, Z. Z., Sarkar, D., Emdad, L., Volsky, D. J., & Fisher, P. B. (2005). Cloning and characterization of HIV-1-inducible astrocyte elevated gene-1, AEG-1. Gene. https://doi.org/10.1016/j.gene.2005.04.006
Vancouver
Kang DC, Su ZZ, Sarkar D, Emdad L, Volsky DJ, Fisher PB. Cloning and characterization of HIV-1-inducible astrocyte elevated gene-1, AEG-1. Gene. 2005. doi:10.1016/j.gene.2005.04.006. PMID: 15927426.
BibTeX
@article{kang2005-15927426,
author = {Kang, D. C. and Su, Z. Z. and Sarkar, D. and Emdad, L. and Volsky, D. J. and Fisher, P. B.},
title = {{Cloning and characterization of HIV-1-inducible astrocyte elevated gene-1, AEG-1}},
journal = {Gene},
year = {2005},
doi = {10.1016/j.gene.2005.04.006},
pmid = {15927426},
url = {https://doi.org/10.1016/j.gene.2005.04.006}
}
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Key findings
- Additionally, a novel method is outlined for producing tag-free recombinant protein in a baculovirus system and its use in producing AEG-1 protein.
- AEG-1 mRNA is expressed ubiquitously with higher expression in tissues containing muscular actin and its expression is increased in astrocytes infected with HIV-1 or treated with gp120 or tumor necrosis factor (TNF)-alpha.
- The mRNA encodes a single pass transmembrane protein of predicted molecular mass of 64-kDa and pI 9.3 that predominantly localizes in the endoplasmic reticulum and perinuclear region.
- Ectopic expression of AEG-1 inhibits excitatory amino acid transporter 2 (EAAT2) promoter activity with the potential to promote glutamate excitotoxicity and consequently HIV-1-associated dementia (HAD).
Why it matters
This study cloned and characterized AEG-1, a gene induced in astrocytes by HIV-1, gp120 or TNF-alpha. It is basic laboratory work related to HIV, not a treatment or cure for Alzheimer's disease.
Study details
- Evidence strengthLow
How this study scored 45/100
Published in a peer-reviewed journal. Scored zero for study design, sample size, effect reported, studied in people and randomized / blinded because the record does not state it.
| Component | Points | Why |
|---|---|---|
| Journal tier | 7 / 10 | Tier 3 — peer-reviewed journal (Gene) |
| Journal's citation record | 3 / 4 | Gene: journal h-index 301 (OpenAlex, 2026-10-29) |
| Study design | 0 / 12 | No study design recorded — scores zero, not guessed (not stated in the record — scored 0) |
| Strength of evidence | 1 / 5 | Rated “low” in our summary |
| Peer-review validation | 10 / 10 | Peer-reviewed. Published as an article in a named peer-reviewed journal. |
| How recent | 1 / 5 | 2005 — older than 20 years, still counted |
| Sample size | 0 / 4 | Participant count not stated — scores zero (not stated in the record — scored 0) |
| Effect reported | 0 / 3 | No effect size reported — scores zero (not stated in the record — scored 0) |
| Studied in people | 0 / 3 | Not stated whether this was studied in people — scores zero (not stated in the record — scored 0) |
| Randomized / blinded | 0 / 2 | No randomization or blinding stated — scores zero (not stated in the record — scored 0) |
| Times cited | 5 / 7 | Cited 287 times (OpenAlex, 2026-10-10) |
| Citations per year | 3 / 5 | 12.5 citations a year over 23 year(s) since 2005 |
| Impact for its field and year | 3 / 6 | Cited 3.0× the average for papers of its field and year (OpenAlex FWCI) |
| Cited by studies here | 0 / 6 | No other study in this library cites it |
| Still being cited | 2 / 4 | Cited 21 times in 2024–2026 (2026 still in progress; OpenAlex) |
| Senior author's track record | 4 / 4 | Last-listed author Paul B. Fisher: h-index 102 (OpenAlex, whole career) |
| Institution's track record | 2 / 3 | Columbia University: institution h-index 1,824 (OpenAlex) |
| Multicenter collaboration | 2 / 3 | OpenAlex lists 5 institution(s) in 2 countries |
| Indexed in PubMed | 2 / 2 | Indexed in PubMed (PMID 15927426) |
| Free to read | 0 / 2 | Behind a paywall — scores zero |
| Total | 45 / 100 | — |
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