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The signaling adapter protein DAP12 regulates multinucleation during osteoclast development
Humphrey MB, Ogasawara K, Yao W, Spusta SC, Daws MR, Lane NE, Lanier LL, Nakamura MC.. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 2003-12-16
Topic: Brain proteins, microglia and lab models
Unlabelled Deficiency of the signaling adapter protein DAP12 is associated with bony abnormalities in both mice and humans. We identify specific DAP12-associated receptors expressed by osteoclasts and examine function of DAP12 in murine osteoclasts in vivo and in vitro. These data show a new role for DAP12 signaling in regulating formation of multinucleated osteoclasts.
Key findings
- We identify specific DAP12-associated receptors expressed by osteoclasts and examine function of DAP12 in murine osteoclasts in vivo and in vitro.
- These data show a new role for DAP12 signaling in regulating formation of multinucleated osteoclasts.
- Introduction Osteoclasts are bone-resorbing cells derived from hematopoietic precursors in the myeloid lineage.
- In other myeloid cell types, the signaling adapter protein DAP12 transmits activating signals on ligation of a DAP12-associated receptor (DAR).
Why it matters
This study found that the signaling protein DAP12 helps regulate the formation of multinucleated osteoclasts, which are bone cells. It concerns bone biology in mice and cells, not Alzheimer's disease, so it does not directly inform Alzheimer's treatment, cure or prevention.
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Study details
- Evidence strengthLow
How this study scored 43/100
Published in a peer-reviewed journal. Scored zero for study design, sample size, effect reported, studied in people and randomised / blinded because the record does not state it.
| Component | Points | Why |
|---|---|---|
| Journal tier | 7 / 10 | Tier 3 — peer-reviewed journal (Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research) |
| Journal's citation record | 3 / 4 | Journal of Bone and Mineral Research: journal h-index 332 (OpenAlex, 2026-10-29) |
| Study design | 0 / 12 | No study design recorded — scores zero, not guessed (not stated in the record — scored 0) |
| Strength of evidence | 1 / 5 | Rated “low” in our summary |
| Peer-review validation | 10 / 10 | Peer-reviewed. Published as an article in a named peer-reviewed journal. |
| How recent | 1 / 5 | 2004 — older than 20 years, still counted |
| Sample size | 0 / 4 | Participant count not stated — scores zero (not stated in the record — scored 0) |
| Effect reported | 0 / 3 | No effect size reported — scores zero (not stated in the record — scored 0) |
| Studied in people | 0 / 3 | Not stated whether this was studied in people — scores zero (not stated in the record — scored 0) |
| Randomised / blinded | 0 / 2 | No randomisation or blinding stated — scores zero (not stated in the record — scored 0) |
| Times cited | 5 / 7 | Cited 124 times (OpenAlex, 2026-10-10) |
| Citations per year | 2 / 5 | 5.2 citations a year over 24 year(s) since 2004 |
| Impact for its field and year | 3 / 6 | Cited 4.0× the average for papers of its field and year (OpenAlex FWCI) |
| Cited by studies here | 2 / 6 | Cited by 8 other studies in this library |
| Still being cited | 1 / 4 | Cited 11 times in 2024–2026 (2026 still in progress; OpenAlex) |
| Senior author's track record | 2 / 4 | Last-listed author Mary C. Nakamura: h-index 47 (OpenAlex, whole career) |
| Institution's track record | 2 / 3 | University of California, San Francisco: institution h-index 1,915 (OpenAlex) |
| Multi-centre collaboration | 2 / 3 | OpenAlex lists 4 institution(s) in 1 country |
| Indexed in PubMed | 2 / 2 | Indexed in PubMed (PMID 14969392) |
| Free to read | 0 / 2 | Behind a paywall — scores zero |
| Total | 43 / 100 | — |
What each of these 20 components measures, and what this score is not →
Read the original
- DOI: 10.1359/jbmr.0301234
- PubMed: 14969392
- Original source →
- Free full text: Journal of Bone and Mineral Research →
Full text: free to read — OpenAlex lists this copy as free to read: the link above (checked 6 Feb 1020).
Cite this study APA, Vancouver, BibTeX · download .bib or .ris
Built from the record alzhub holds for this paper, not looked up. Volume, issue and page numbers are not held in the record. The record names 8 authors; APA and Vancouver show the first six, BibTeX and the downloads carry all of them. Check the citation against the original before you use it.
APA 7th
Humphrey, M. B., Ogasawara, K., Yao, W., Spusta, S. C., Daws, M. R., Lane, N. E., et al. (2004). The signaling adapter protein DAP12 regulates multinucleation during osteoclast development. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. https://doi.org/10.1359/jbmr.0301234
Vancouver
Humphrey MB, Ogasawara K, Yao W, Spusta SC, Daws MR, Lane NE, et al. The signaling adapter protein DAP12 regulates multinucleation during osteoclast development. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 2004. doi:10.1359/jbmr.0301234. PMID: 14969392.
BibTeX
@article{humphrey2004-14969392,
author = {Humphrey, M. B. and Ogasawara, K. and Yao, W. and Spusta, S. C. and Daws, M. R. and Lane, N. E. and Lanier, L. L. and Nakamura, M. C.},
title = {{The signaling adapter protein DAP12 regulates multinucleation during osteoclast development}},
journal = {Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research},
year = {2004},
doi = {10.1359/jbmr.0301234},
pmid = {14969392},
url = {https://doi.org/10.1359/jbmr.0301234}
}
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