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Protein phosphorylation regulates secretion of Alzheimer beta/A4 amyloid precursor protein
Caporaso GL, Gandy SE, Buxbaum JD, Ramabhadran TV, Greengard P.. Proceedings of the National Academy of Sciences of the United States of America, 1992-04-01
Topic: Brain proteins, microglia and lab models
Extracellular deposition of the beta/A4 amyloid peptide is a characteristic feature of the brain in patients with Alzheimer disease. beta/A4 amyloid is derived from the amyloid precursor protein (APP), an integral membrane protein that exists as three major isoforms (APP695, APP751, and APP770). Secreted forms of APP found in blood plasma and cerebrospinal fluid arise by proteolytic cleavage of APP within the beta/A4 amyloid domain, precluding the possibility of amyloidogenesis for that population of molecules. In the present study, we have demonstrated that treatment of PC12 cells with phorbol ester produces a severalfold increase in secretion of APP695, APP751, and APP770.
Cite this study APA, Vancouver, BibTeX · download .bib or .ris
APA 7th
Caporaso, G. L., Gandy, S. E., Buxbaum, J. D., Ramabhadran, T. V., & Greengard, P. (1992). Protein phosphorylation regulates secretion of Alzheimer beta/A4 amyloid precursor protein. Proceedings of the National Academy of Sciences of the United States of America. https://doi.org/10.1073/pnas.89.7.3055
Vancouver
Caporaso GL, Gandy SE, Buxbaum JD, Ramabhadran TV, Greengard P. Protein phosphorylation regulates secretion of Alzheimer beta/A4 amyloid precursor protein. Proceedings of the National Academy of Sciences of the United States of America. 1992. doi:10.1073/pnas.89.7.3055. PMID: 1557413.
BibTeX
@article{caporaso1992-1557413,
author = {Caporaso, G. L. and Gandy, S. E. and Buxbaum, J. D. and Ramabhadran, T. V. and Greengard, P.},
title = {{Protein phosphorylation regulates secretion of Alzheimer beta/A4 amyloid precursor protein}},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
year = {1992},
doi = {10.1073/pnas.89.7.3055},
pmid = {1557413},
url = {https://doi.org/10.1073/pnas.89.7.3055}
}
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Key findings
- Secreted forms of APP found in blood plasma and cerebrospinal fluid arise by proteolytic cleavage of APP within the beta/A4 amyloid domain, precluding the possibility of amyloidogenesis for that population of molecules.
- In the present study, we have demonstrated that treatment of PC12 cells with phorbol ester produces a severalfold increase in secretion of APP695, APP751, and APP770.
- This increase is augmented by simultaneous treatment with the protein phosphatase inhibitor okadaic acid.
- These data indicate that protein phosphorylation regulates intra-beta/A4 amyloid cleavage and APP secretion.
Why it matters
In PC12 cells, phorbol ester treatment increased secretion of APP695, APP751 and APP770, and this increase was augmented by okadaic acid. This is laboratory research and does not test a treatment or cure for Alzheimer's disease.
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Study details
- Evidence strengthLow
How this study scored 50/100
Published in a peer-reviewed journal. Scored zero for study design, sample size, effect reported, studied in people and randomised / blinded because the record does not state it.
| Component | Points | Why |
|---|---|---|
| Journal tier | 7 / 10 | Tier 3 — peer-reviewed journal (Proceedings of the National Academy of Sciences of the United States of America) |
| Journal's citation record | 4 / 4 | Proceedings of the National Academy of Sciences: journal h-index 1,167 (OpenAlex, 2026-10-29) |
| Study design | 0 / 12 | No study design recorded — scores zero, not guessed (not stated in the record — scored 0) |
| Strength of evidence | 1 / 5 | Rated “low” in our summary |
| Peer-review validation | 10 / 10 | Peer-reviewed. Published as an article in a named peer-reviewed journal. |
| How recent | 1 / 5 | 1992 — older than 20 years, still counted |
| Sample size | 0 / 4 | Participant count not stated — scores zero (not stated in the record — scored 0) |
| Effect reported | 0 / 3 | No effect size reported — scores zero (not stated in the record — scored 0) |
| Studied in people | 0 / 3 | Not stated whether this was studied in people — scores zero (not stated in the record — scored 0) |
| Randomised / blinded | 0 / 2 | No randomisation or blinding stated — scores zero (not stated in the record — scored 0) |
| Times cited | 6 / 7 | Cited 340 times (OpenAlex, 2026-10-10) |
| Citations per year | 3 / 5 | 9.4 citations a year over 36 year(s) since 1992 |
| Impact for its field and year | 5 / 6 | Cited 20.7× the average for papers of its field and year (OpenAlex FWCI) |
| Cited by studies here | 3 / 6 | Cited by 14 other studies in this library |
| Still being cited | 0 / 4 | Cited 1 times in 2024–2026 (2026 still in progress; OpenAlex) |
| Senior author's track record | 4 / 4 | Last-listed author Paul Greengard: h-index 206 (OpenAlex, whole career) |
| Institution's track record | 2 / 3 | New York University: institution h-index 1,612 (OpenAlex) |
| Multi-centre collaboration | 2 / 3 | OpenAlex lists 4 institution(s) in 1 country |
| Indexed in PubMed | 2 / 2 | Indexed in PubMed (PMID 1557413) |
| Free to read | 0 / 2 | Behind a paywall — scores zero |
| Total | 50 / 100 | — |
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