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Development of Biotin and Horseradish Peroxidase Labelling against Monoclonal Antibody Amyloid-β42 and Its Application on Enzyme-Linked Immunosorbent Assay
Nur Rizky Fiero, Uus Saepuloh, Huda Shalahudin Darusman, Rachmitasari Noviana. Jurnal Penelitian Pendidikan IPA, 2025-10-25
Topic: Brain proteins, microglia and lab models
Alzheimer disease is one of the causes of dementia that could make the patient lose their memories until they unable to do normal activities. It is caused by accumulation of protein in the brain called Amyloid-β42 (Aβ42), that often used as biomarker for alzheimer by enzyme-linked immunosorbent assay (ELISA). To increase the sensitivity and accuracy, capture antibodies are conjugated with labels such as Horseradish Peroxidase (HRP) and Biotin. However, the two conjugates also differ in sensitivity and specificity, therefore this research is to find which is more optimum and stable for ELISA usage. The molecular mass of the conjugated antibodies was characterized with SDS-PAGE, result shows the pre-conjugated antibody has 150 kDa bands, conjugated antibody with labels is heavier than the pre-conjugated due to the addition of each label. Conjugated antibody tested with tetramethylbenzidine (TMB), then analyzed by ELISA reader. The measurement precision decided with %CV value with results shows dillution from 10-1 to 10-4 has %CV less than 10%, while Biotin is from 10-1 to 10-5. Optimization done by determining fixed antigen with the conjugated antibody, the optimal concentration for HRP is 10-3 while biotin is at 10-4. Stability of the conjugated antibody was also determined, HRP measurement started to unstable around second month and third month, while biotin start to unstable around fourth month, this shows the biotin is more stable than the HRP.
Cite this study APA, Vancouver, BibTeX · download .bib or .ris
APA 7th
Fiero, N. R., Saepuloh, U., Darusman, H. S., & Noviana, R. (2025). Development of Biotin and Horseradish Peroxidase Labelling against Monoclonal Antibody Amyloid-β42 and Its Application on Enzyme-Linked Immunosorbent Assay. Jurnal Penelitian Pendidikan IPA. https://doi.org/10.29303/jppipa.v11i10.12528
Vancouver
Fiero NR, Saepuloh U, Darusman HS, Noviana R. Development of Biotin and Horseradish Peroxidase Labelling against Monoclonal Antibody Amyloid-β42 and Its Application on Enzyme-Linked Immunosorbent Assay. Jurnal Penelitian Pendidikan IPA. 2025. doi:10.29303/jppipa.v11i10.12528.
BibTeX
@article{fiero2025-65f4fa6e,
author = {Fiero, Nur Rizky and Saepuloh, Uus and Darusman, Huda Shalahudin and Noviana, Rachmitasari},
title = {{Development of Biotin and Horseradish Peroxidase Labelling against Monoclonal Antibody Amyloid-β42 and Its Application on Enzyme-Linked Immunosorbent Assay}},
journal = {Jurnal Penelitian Pendidikan IPA},
year = {2025},
doi = {10.29303/jppipa.v11i10.12528},
url = {https://doi.org/10.29303/jppipa.v11i10.12528}
}
Built from the record alzhub holds for this paper, not looked up. Volume, issue and page numbers are not held in the record. Check the citation against the original before you use it.
Key findings
- The molecular mass of the conjugated antibodies was characterized with SDS-PAGE, result shows the pre-conjugated antibody has 150 kDa bands, conjugated antibody with labels is heavier than the pre-conjugated due to the addition of each label.
- The measurement precision decided with %CV value with results shows dillution from 10-1 to 10-4 has %CV less than 10%, while Biotin is from 10-1 to 10-5.
Why it matters
This laboratory work compared two antibody labelling methods, horseradish peroxidase and biotin, for use in an ELISA test that measures the Alzheimer's-related protein Amyloid-β42. It is a technical methods study about test reagents and does not test any treatment, prevention or cure in patients.
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Study details
- Evidence strengthLow
How this study scored 25/100
Published in a peer-reviewed journal. Scored zero for study design, sample size, effect reported, studied in people and randomised / blinded because the record does not state it.
| Component | Points | Why |
|---|---|---|
| Journal tier | 7 / 10 | Tier 3 — peer-reviewed journal (Jurnal Penelitian Pendidikan IPA) |
| Journal's citation record | 0 / 4 | Jurnal Penelitian Pendidikan IPA: journal h-index 35 (OpenAlex, 2026-10-29) |
| Study design | 0 / 12 | No study design recorded — scores zero, not guessed (not stated in the record — scored 0) |
| Strength of evidence | 1 / 5 | Rated “low” in our summary |
| Peer-review validation | 10 / 10 | Peer-reviewed. Published as an article in a named peer-reviewed journal. |
| How recent | 4 / 5 | 2025 — within 3 year(s) of the newest study here |
| Sample size | 0 / 4 | Participant count not stated — scores zero (not stated in the record — scored 0) |
| Effect reported | 0 / 3 | No effect size reported — scores zero (not stated in the record — scored 0) |
| Studied in people | 0 / 3 | Not stated whether this was studied in people — scores zero (not stated in the record — scored 0) |
| Randomised / blinded | 0 / 2 | No randomisation or blinding stated — scores zero (not stated in the record — scored 0) |
| Times cited | 0 / 7 | Cited 0 times (OpenAlex, 2026-10-10) |
| Citations per year | 0 / 5 | 0.0 citations a year over 3 year(s) since 2025 |
| Impact for its field and year | 0 / 6 | Cited 0.0× the average for papers of its field and year (OpenAlex FWCI) |
| Cited by studies here | 0 / 6 | No other study in this library cites it |
| Still being cited | 0 / 4 | Cited 0 times in 2024–2026 (2026 still in progress; OpenAlex) |
| Senior author's track record | 0 / 4 | Last-listed author Rachmitasari Noviana: h-index 3 (OpenAlex, whole career) |
| Institution's track record | 0 / 3 | IPB University: institution h-index 181 (OpenAlex) |
| Multi-centre collaboration | 0 / 3 | OpenAlex lists 1 institution(s) in 1 country — scores zero (affiliation lists can be incomplete) |
| Indexed in PubMed | 1 / 2 | Not in PubMed, but has a DOI |
| Free to read | 2 / 2 | Free to read |
| Total | 25 / 100 | — |
What each of these 20 components measures, and what this score is not →
Read the original
- DOI: 10.29303/jppipa.v11i10.12528
- Original source →
- Free full text: Jurnal Penelitian Pendidikan IPA →
Full text: free to read — OpenAlex lists this copy as free to read: the link above (checked 6 Feb 1020).
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