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A novel silanization agent based single used biosensing system: Detection of C-reactive protein as a potential Alzheimer's disease blood biomarker
Sonuç Karaboğa MN, Sezgintürk MK.. Journal of pharmaceutical and biomedical analysis, 2018-03-12
Topic: Amyloid, tau and blood-test diagnosis
This paper illustrates a new and sensitive electrochemical immunosensor for the analysis of C-reactive protein. Indium Tin Oxide (ITO) disposable sheets were modified by using 3-cyanopropyltrimethoxysilane (CPTMS) self-assembled monolayers (SAMs) for the first time for immobilizing the anti-CRP antibody via covalent interactions without the need for any cross-linking agent. Cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS), as well as square wave voltammetry (SWV) methods were applied to characterize immobilization steps of anti-CRP and to determine the CRP concentration. The optimization of the fabricated parameters and the analytical performance of the biosensor were widely evaluated. Charge transfer resistance changes were highly linear and sensitive with CRP concentration of 3.25-208 fg mL -1 range and associated with a limit of detection of 0.455 fg mL -1 . This impedimetric biosensing system have excellent repeatability, reproducibility and reusability. Moreover, the binding characterization of CRP to anti-CRP was monitored by a single frequency impedance technique. The amount of CRP in human serum samples were analyzed by fabricated biosensor to determine the feasibility of the biosensing system in medical purposes. We suggest that CPTMS, a new silanization agent, is ideal in biosensor applications.
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APA 7th
Sonuç Karaboğa, M. N., & Sezgintürk, M. K. (2018). A novel silanization agent based single used biosensing system: Detection of C-reactive protein as a potential Alzheimer's disease blood biomarker. Journal of pharmaceutical and biomedical analysis. https://doi.org/10.1016/j.jpba.2018.03.016
Vancouver
Sonuç Karaboğa MN, Sezgintürk MK. A novel silanization agent based single used biosensing system: Detection of C-reactive protein as a potential Alzheimer's disease blood biomarker. Journal of pharmaceutical and biomedical analysis. 2018. doi:10.1016/j.jpba.2018.03.016. PMID: 29558723.
BibTeX
@article{sonuckaraboga2018-29558723,
author = {Sonuç Karaboğa, M. N. and Sezgintürk, M. K.},
title = {{A novel silanization agent based single used biosensing system: Detection of C-reactive protein as a potential Alzheimer's disease blood biomarker}},
journal = {Journal of pharmaceutical and biomedical analysis},
year = {2018},
doi = {10.1016/j.jpba.2018.03.016},
pmid = {29558723},
url = {https://doi.org/10.1016/j.jpba.2018.03.016}
}
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Key findings
- Charge transfer resistance changes were highly linear and sensitive with CRP concentration of 3.25-208 fg mL -1 range and associated with a limit of detection of 0.455 fg mL -1 .
- We suggest that CPTMS, a new silanization agent, is ideal in biosensor applications.
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Study details
- Evidence strengthLow
How this study scored 38/100
Published in a peer-reviewed journal. Scored zero for study design, sample size, effect reported, studied in people and randomised / blinded because the record does not state it. We have no data on journal's citation record, times cited, citations per year, impact for its field and year, cited by studies here, still being cited, senior author's track record, institution's track record and multi-centre collaboration, so those were left out: it earned 22 of the 58 points we could judge it on, which is 38 out of 100.
| Component | Points | Why |
|---|---|---|
| Journal tier | 7 / 10 | Tier 3 — peer-reviewed journal (Journal of pharmaceutical and biomedical analysis) |
| Journal's citation record | — / 4 | No journal record from OpenAlex — left out of this score |
| Study design | 0 / 12 | No study design recorded — scores zero, not guessed (not stated in the record — scored 0) |
| Strength of evidence | 1 / 5 | Rated “low” in our summary |
| Peer-review validation | 10 / 10 | Peer-reviewed. Published as an article in a named peer-reviewed journal. |
| How recent | 2 / 5 | 2018 — within 10 year(s) of the newest study here |
| Sample size | 0 / 4 | Participant count not stated — scores zero (not stated in the record — scored 0) |
| Effect reported | 0 / 3 | No effect size reported — scores zero (not stated in the record — scored 0) |
| Studied in people | 0 / 3 | Not stated whether this was studied in people — scores zero (not stated in the record — scored 0) |
| Randomised / blinded | 0 / 2 | No randomisation or blinding stated — scores zero (not stated in the record — scored 0) |
| Times cited | — / 7 | No citation count (not found in OpenAlex) — left out of this score |
| Citations per year | — / 5 | No citation count or no year — left out of this score |
| Impact for its field and year | — / 6 | No field-weighted citation impact (OpenAlex has none yet) — left out of this score |
| Cited by studies here | — / 6 | Not yet checked against this library's citation graph — left out of this score |
| Still being cited | — / 4 | No yearly citation counts — left out of this score |
| Senior author's track record | — / 4 | Senior author's record not available — left out of this score |
| Institution's track record | — / 3 | No institution listed in OpenAlex — left out of this score |
| Multi-centre collaboration | — / 3 | Institutions not listed — left out of this score |
| Indexed in PubMed | 2 / 2 | Indexed in PubMed (PMID 29558723) |
| Free to read | 0 / 2 | Behind a paywall — scores zero |
| Points | 22 / 58 | Scored on 11 of 20 parts. We have no data for the rest, so they are left out: 22 of the 58 points we could judge it on is 38 out of 100. |
| Total | 38 / 100 | — |
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