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39/100

✓ Peer-reviewed alzhub usefulness score 39 out of 100. Biomarkers

Topic: Amyloid, tau and blood-test diagnosis

Alzheimer disease plasma biomarkers have emerged as minimally invasive, cost-effective tools for early diagnosis and disease monitoring yet their stability under common "real world" pre-analytical conditions remains incompletely characterized. We evaluated the stability of six plasma biomarkers, Aβ40, Aβ42, pTau181, pTau217, NfL, and glial fibrillary acidic protein (GFAP) using the Fujirebio Lumipulse G1200 platform. Plasma samples were initially collected from four healthy and cognitively unimpaired volunteers. Samples were stored under four conditions: room temperature (0-4 h), +4 °C (1-10 days), -20 °C (1-3 weeks), and -80 °C (4-8 weeks). In this pilot study, Aβ40 and Aβ42 remained generally stable. In contrast, pTau181 readings exhibited marked elevations in frozen samples, while pTau217 showed modest early fluctuations followed by significant decreases with prolonged storage. Next, we recruited 12 additional participants (six cognitively normal and six with mild cognitive impairment [MCI]), and their plasma samples were analyzed both fresh and after 4 weeks of storage at -80 °C. Among these participants, pTau181 readouts were significantly higher, and pTau217 were lower, in -80 °C frozen in comparison to never-frozen samples. These findings underscore the critical need for biomarker-specific sample workup and handling protocols and indicate that results for fresh plasma cannot be assumed to be the same as for frozen samples.

Cite this study APA, Vancouver, BibTeX · download .bib or .ris

APA 7th

Choucair, I., Lee, T. L., Van Eldik, L. J., Dage, J. L., Gold, B. T., Abner, E. L., et al. (2025). Storage-dependent variability in Alzheimer disease-related plasma biomarker results using the Fujirebio Lumipulse G1200 platform. Journal of Neuropathology & Experimental Neurology. https://doi.org/10.1093/jnen/nlaf115

Vancouver

Choucair I, Lee TL, Van Eldik LJ, Dage JL, Gold BT, Abner EL, et al. Storage-dependent variability in Alzheimer disease-related plasma biomarker results using the Fujirebio Lumipulse G1200 platform. Journal of Neuropathology & Experimental Neurology. 2025. doi:10.1093/jnen/nlaf115. PMID: 41069044.

BibTeX

@article{choucair2025-41069044,
  author  = {Choucair, Ibrahim and Lee, Tiffany L. and Van Eldik, Linda J. and Dage, Jeffrey L. and Gold, Brian T. and Abner, Erin L. and Jicha, Gregory A. and Nelson, Peter T.},
  title   = {{Storage-dependent variability in Alzheimer disease-related plasma biomarker results using the Fujirebio Lumipulse G1200 platform}},
  journal = {Journal of Neuropathology \& Experimental Neurology},
  year    = {2025},
  doi     = {10.1093/jnen/nlaf115},
  pmid    = {41069044},
  url     = {https://doi.org/10.1093/jnen/nlaf115}
}

Built from the record alzhub holds for this paper, not looked up. Volume, issue and page numbers are not held in the record. The record names 8 authors; APA and Vancouver show the first six, BibTeX and the downloads carry all of them. Check the citation against the original before you use it.

Key findings

In contrast, pTau181 readings exhibited marked elevations in frozen samples, while pTau217 showed modest early fluctuations followed by significant decreases with prolonged storage.
Among these participants, pTau181 readouts were significantly higher, and pTau217 were lower, in -80 °C frozen in comparison to never-frozen samples.
These findings underscore the critical need for biomarker-specific sample workup and handling protocols and indicate that results for fresh plasma cannot be assumed to be the same as for frozen samples.

Why it matters

In this small pilot, pTau181 rose and pTau217 fell in frozen compared with fresh plasma samples, while Aβ40 and Aβ42 were generally stable. This is a sample-handling caution and does not address treatment, cure or prevention.

New to this subject? Start with our plain-English guide: Blood tests & biomarkers →

Study details

Participants12
Evidence strengthLow

How this study scored 39/100

Published in a peer-reviewed journal. Scored zero for study design, effect reported and randomised / blinded because the record does not state it.

Component	Points	Why
Journal tier	7 / 10	Tier 3 — peer-reviewed journal (Journal of Neuropathology & Experimental Neurology)
Journal's citation record	2 / 4	Journal of Neuropathology & Experimental Neurology: journal h-index 239 (OpenAlex, 2026-10-29)
Study design	0 / 12	No study design recorded — scores zero, not guessed (not stated in the record — scored 0)
Strength of evidence	1 / 5	Rated “low” in our summary
Peer-review validation	10 / 10	Peer-reviewed. Published as an article in a named peer-reviewed journal.
How recent	4 / 5	2025 — within 3 year(s) of the newest study here
Sample size	1 / 4	12 participants
Effect reported	0 / 3	No effect size reported — scores zero (not stated in the record — scored 0)
Studied in people	3 / 3	Studied in people (participant count stated)
Randomised / blinded	0 / 2	No randomisation or blinding stated — scores zero (not stated in the record — scored 0)
Times cited	1 / 7	Cited 1 times (OpenAlex, 2026-10-10)
Citations per year	0 / 5	0.3 citations a year over 3 year(s) since 2025
Impact for its field and year	1 / 6	Cited 0.5× the average for papers of its field and year (OpenAlex FWCI)
Cited by studies here	0 / 6	No other study in this library cites it
Still being cited	0 / 4	Cited 1 times in 2024–2026 (2026 still in progress; OpenAlex)
Senior author's track record	3 / 4	Last-listed author Peter T. Nelson: h-index 92 (OpenAlex, whole career)
Institution's track record	1 / 3	University of Kentucky: institution h-index 824 (OpenAlex)
Multi-centre collaboration	1 / 3	OpenAlex lists 2 institution(s) in 1 country
Indexed in PubMed	2 / 2	Indexed in PubMed (PMID 41069044)
Free to read	2 / 2	Free to read
Total	39 / 100	—

What each of these 20 components measures, and what this score is not →

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