Home › Research › Tau biomarkers
Importance and impact of preanalytical variables on Alzheimer disease biomarker concentrations in cerebrospinal fluid
Le Bastard N, De Deyn PP, Engelborghs S.. Clinical chemistry, 2015-04-13
Topic: Amyloid, tau and blood-test diagnosis
Background Analyses of cerebrospinal fluid (CSF) biomarkers (β-amyloid protein, total tau protein, and hyperphosphorylated tau protein) are part of the diagnostic criteria of Alzheimer disease. Different preanalytical sample procedures contribute to variability of CSF biomarker concentrations, hampering between-laboratory comparisons. The aim of this study was to explore the influence of fractionated sampling, centrifugation, freezing temperature, freezing delay, and freeze-thaw cycles on CSF biomarker analyses.
Cite this study APA, Vancouver, BibTeX · download .bib or .ris
APA 7th
Le Bastard, N., De Deyn, P. P., & Engelborghs, S. (2015). Importance and impact of preanalytical variables on Alzheimer disease biomarker concentrations in cerebrospinal fluid. Clinical chemistry. https://doi.org/10.1373/clinchem.2014.236679
Vancouver
Le Bastard N, De Deyn PP, Engelborghs S. Importance and impact of preanalytical variables on Alzheimer disease biomarker concentrations in cerebrospinal fluid. Clinical chemistry. 2015. doi:10.1373/clinchem.2014.236679. PMID: 25869575.
BibTeX
@article{lebastard2015-25869575,
author = {Le Bastard, N. and De Deyn, P. P. and Engelborghs, S.},
title = {{Importance and impact of preanalytical variables on Alzheimer disease biomarker concentrations in cerebrospinal fluid}},
journal = {Clinical chemistry},
year = {2015},
doi = {10.1373/clinchem.2014.236679},
pmid = {25869575},
url = {https://doi.org/10.1373/clinchem.2014.236679}
}
Built from the record alzhub holds for this paper, not looked up. Volume, issue and page numbers are not held in the record. Check the citation against the original before you use it.
Key findings
- Different preanalytical sample procedures contribute to variability of CSF biomarker concentrations, hampering between-laboratory comparisons.
- The aim of this study was to explore the influence of fractionated sampling, centrifugation, freezing temperature, freezing delay, and freeze-thaw cycles on CSF biomarker analyses.
- Methods We studied fractionated sampling in sequential aliquots of lumbar CSF.
- Centrifuged and noncentrifuged samples from the same fraction were compared.
Why it matters
This study examined how different sample handling procedures before analysis affect cerebrospinal fluid Alzheimer's biomarker concentrations. It is a laboratory methods study and does not test any treatment, cure, or prevention.
New to this subject? Start with our plain-English guide: Blood tests & biomarkers →
Study details
- Evidence strengthLow
How this study scored 42/100
Published in a peer-reviewed journal. Scored zero for study design, sample size, effect reported, studied in people and randomized / blinded because the record does not state it.
| Component | Points | Why |
|---|---|---|
| Journal tier | 7 / 10 | Tier 3 — peer-reviewed journal (Clinical chemistry) |
| Journal's citation record | 3 / 4 | Clinical Chemistry: journal h-index 323 (OpenAlex, 2026-10-29) |
| Study design | 0 / 12 | No study design recorded — scores zero, not guessed (not stated in the record — scored 0) |
| Strength of evidence | 1 / 5 | Rated “low” in our summary |
| Peer-review validation | 10 / 10 | Peer-reviewed. Published as an article in a named peer-reviewed journal. |
| How recent | 1 / 5 | 2015 — within 20 year(s) of the newest study here |
| Sample size | 0 / 4 | Participant count not stated — scores zero (not stated in the record — scored 0) |
| Effect reported | 0 / 3 | No effect size reported — scores zero (not stated in the record — scored 0) |
| Studied in people | 0 / 3 | Not stated whether this was studied in people — scores zero (not stated in the record — scored 0) |
| Randomized / blinded | 0 / 2 | No randomization or blinding stated — scores zero (not stated in the record — scored 0) |
| Times cited | 4 / 7 | Cited 69 times (OpenAlex, 2026-10-10) |
| Citations per year | 2 / 5 | 5.3 citations a year over 13 year(s) since 2015 |
| Impact for its field and year | 3 / 6 | Cited 6.9× the average for papers of its field and year (OpenAlex FWCI) |
| Cited by studies here | 2 / 6 | Cited by 3 other studies in this library |
| Still being cited | 1 / 4 | Cited 11 times in 2024–2026 (2026 still in progress; OpenAlex) |
| Senior author's track record | 3 / 4 | Last-listed author Sebastiaan Engelborghs: h-index 95 (OpenAlex, whole career) |
| Institution's track record | 1 / 3 | University Medical Center Groningen: institution h-index 995 (OpenAlex) |
| Multicenter collaboration | 2 / 3 | OpenAlex lists 4 institution(s) in 2 countries |
| Indexed in PubMed | 2 / 2 | Indexed in PubMed (PMID 25869575) |
| Free to read | 0 / 2 | Behind a paywall — scores zero |
| Total | 42 / 100 | — |
What each of these 20 components measures, and what this score is not →
Read the original
May need a subscription OpenAlex found no free copy of the full text (checked 6 Feb 1020), so the publisher may charge for it. The PubMed abstract is free.
Related
Studies in the library that share something with this one. The line above each title says what.
Studies this one cites
Referenced by this study and also in the alzhub library, so you can read them here.
Later studies that cite this one
3 studies in the alzhub library list this one in their references.
Related studies in Tau biomarkers
Also tagged “cerebrospinal”
820 studies in the library carry the topic tag “cerebrospinal”; these are this study's neighbours in a fixed, alphabetical-by-hash tour of them, not the closest in content. All 820 tagged studies.