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CSF Biomarkers in Longitudinal Alzheimer Disease Cohorts: Pre-Analytic Challenges
Jonaitis EM, Jeffers B, VandenLangenberg M, Ma Y, Van Hulle C, Langhough R, Du L, Chin NA et al.. Clinical chemistry, 2024-03-01
Topic: Amyloid, tau and blood-test diagnosis
Background The sensitivity of amyloid to pre-analytic factors complicates cerebrospinal fluid (CSF) diagnostics for Alzheimer disease. We report reliability and validity evidence for automated immunoassays from frozen and fresh CSF samples in an ongoing, single-site research program. Methods CSF samples were obtained from 2 Wisconsin cohorts (1256 measurements; 727 participants).
Cite this study APA, Vancouver, BibTeX · download .bib or .ris
APA 7th
Jonaitis, E. M., Jeffers, B., VandenLangenberg, M., Ma, Y., Van Hulle, C., Langhough, R., et al. (2024). CSF Biomarkers in Longitudinal Alzheimer Disease Cohorts: Pre-Analytic Challenges. Clinical chemistry. https://doi.org/10.1093/clinchem/hvad221
Vancouver
Jonaitis EM, Jeffers B, VandenLangenberg M, Ma Y, Van Hulle C, Langhough R, et al. CSF Biomarkers in Longitudinal Alzheimer Disease Cohorts: Pre-Analytic Challenges. Clinical chemistry. 2024. doi:10.1093/clinchem/hvad221. PMID: 38431278.
BibTeX
@article{jonaitis2024-38431278,
author = {Jonaitis, E. M. and Jeffers, B. and VandenLangenberg, M. and Ma, Y. and Van Hulle, C. and Langhough, R. and Du, L. and Chin, N. A. and others},
title = {{CSF Biomarkers in Longitudinal Alzheimer Disease Cohorts: Pre-Analytic Challenges}},
journal = {Clinical chemistry},
year = {2024},
doi = {10.1093/clinchem/hvad221},
pmid = {38431278},
url = {https://doi.org/10.1093/clinchem/hvad221}
}
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Key findings
- We report reliability and validity evidence for automated immunoassays from frozen and fresh CSF samples in an ongoing, single-site research program.
- Methods CSF samples were obtained from 2 Wisconsin cohorts (1256 measurements; 727 participants).
- Levels of amyloid beta 1-42 (Aβ42), phosphorylated tau 181 (pTau181), and total tau (tTau) were obtained using an Elecsys cobas e 601 platform.
- Repeatability and fixed effects of storage tube type, extraction method, and freezing were assessed via mixed models.
Why it matters
This study examined how handling and storage of spinal fluid samples affect the reliability of Alzheimer's biomarker measurements. It is about laboratory methods and quality control, not about treating or preventing the disease.
New to this subject? Start with our plain-English guide: Blood tests & biomarkers →
Study details
- Study designlongitudinal study
- Participants727
- Evidence strengthModerate
How this study scored 56/100
Published in a peer-reviewed journal, and it is a longitudinal study following the same people over time. Scored zero for effect reported and randomized / blinded because the record does not state it.
| Component | Points | Why |
|---|---|---|
| Journal tier | 7 / 10 | Tier 3 — peer-reviewed journal (Clinical chemistry) |
| Journal's citation record | 3 / 4 | Clinical Chemistry: journal h-index 323 (OpenAlex, 2026-10-29) |
| Study design | 7 / 12 | A longitudinal study following the same people over time |
| Strength of evidence | 3 / 5 | Rated “moderate” in our summary |
| Peer-review validation | 10 / 10 | Peer-reviewed. Published as an article in a named peer-reviewed journal. |
| How recent | 4 / 5 | 2024 — within 3 year(s) of the newest study here |
| Sample size | 2 / 4 | 727 participants |
| Effect reported | 0 / 3 | No effect size reported — scores zero (not stated in the record — scored 0) |
| Studied in people | 3 / 3 | Studied in people (participant count stated) |
| Randomized / blinded | 0 / 2 | No randomization or blinding stated — scores zero (not stated in the record — scored 0) |
| Times cited | 2 / 7 | Cited 9 times (OpenAlex, 2026-10-10) |
| Citations per year | 1 / 5 | 2.2 citations a year over 4 year(s) since 2024 |
| Impact for its field and year | 2 / 6 | Cited 2.4× the average for papers of its field and year (OpenAlex FWCI) |
| Cited by studies here | 0 / 6 | No other study in this library cites it |
| Still being cited | 1 / 4 | Cited 9 times in 2024–2026 (2026 still in progress; OpenAlex) |
| Senior author's track record | 4 / 4 | Last-listed author Sterling C. Johnson: h-index 112 (OpenAlex, whole career) |
| Institution's track record | 2 / 3 | University of Wisconsin–Madison: institution h-index 1,518 (OpenAlex) |
| Multicenter collaboration | 1 / 3 | OpenAlex lists 2 institution(s) in 1 country |
| Indexed in PubMed | 2 / 2 | Indexed in PubMed (PMID 38431278) |
| Free to read | 2 / 2 | Free to read |
| Total | 56 / 100 | — |
What each of these 20 components measures, and what this score is not →
Read the original
- DOI: 10.1093/clinchem/hvad221
- PubMed: 38431278
- Original source →
- Free full text: Clinical Chemistry →
Full text: free to read — OpenAlex lists this copy as free to read: the link above (checked 6 Feb 1020).
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