Home › Research › Prevention trials
Structural Basis for Chaperone-Independent Ubiquitination of Tau Protein by Its E3 Ligase CHIP
Francesca Munari, Luca Mollica, Carlo Valente, Francesca Parolini, Elham Ataie Kachoie, Giorgio Arrigoni, Mariapina D'Onofrio, Stefano Capaldi et al.. Angewandte Chemie (International ed. in English), 2022-02-18
Topic: Disease mechanisms in lab and animal studies
The multi-site ubiquitination of Tau protein found in Alzheimer's disease filaments hints at the failed attempt of neurons to remove early toxic species. The ubiquitin-dependent degradation of Tau is regulated in vivo by the E3 ligase CHIP, a quality controller of the cell proteome dedicated to target misfolded proteins for degradation. In our study, by using site-resolved NMR, biochemical and computational methods, we elucidate the structural determinants underlying the molecular recognition between the ligase and its intrinsically disordered substrate. We reveal a multi-domain dynamic interaction that explains how CHIP can direct ubiquitination of Tau at multiple sites even in the absence of chaperones, including its typical partner Hsp70/Hsc70. Our findings thus provide mechanistic insight into the chaperone-independent engagement of a disordered protein by its E3 ligase.
Cite this study APA, Vancouver, BibTeX · download .bib or .ris
APA 7th
Munari, F., Mollica, L., Valente, C., Parolini, F., Kachoie, E. A., Arrigoni, G., et al. (2022). Structural Basis for Chaperone-Independent Ubiquitination of Tau Protein by Its E3 Ligase CHIP. Angewandte Chemie (International ed. in English). https://doi.org/10.1002/anie.202112374
Vancouver
Munari F, Mollica L, Valente C, Parolini F, Kachoie EA, Arrigoni G, et al. Structural Basis for Chaperone-Independent Ubiquitination of Tau Protein by Its E3 Ligase CHIP. Angewandte Chemie (International ed. in English). 2022. doi:10.1002/anie.202112374. PMID: 35107860.
BibTeX
@article{munari2022-35107860,
author = {Munari, Francesca and Mollica, Luca and Valente, Carlo and Parolini, Francesca and Kachoie, Elham Ataie and Arrigoni, Giorgio and D'Onofrio, Mariapina and Capaldi, Stefano and others},
title = {{Structural Basis for Chaperone-Independent Ubiquitination of Tau Protein by Its E3 Ligase CHIP}},
journal = {Angewandte Chemie (International ed. in English)},
year = {2022},
doi = {10.1002/anie.202112374},
pmid = {35107860},
url = {https://doi.org/10.1002/anie.202112374}
}
Built from the record alzhub holds for this paper, not looked up. Volume, issue and page numbers are not held in the record. The record's author list ends in “et al.”, so not every author is named. Check the citation against the original before you use it.
Key findings
- Our findings thus provide mechanistic insight into the chaperone-independent engagement of a disordered protein by its E3 ligase.
New to this subject? Start with our plain-English guide: Prevention →
Study details
- Evidence strengthLow
How this study scored 41/100
Published in a peer-reviewed journal. Scored zero for study design, sample size, effect reported, studied in people and randomised / blinded because the record does not state it. We have no data on journal's citation record, times cited, citations per year, impact for its field and year, cited by studies here, still being cited, senior author's track record, institution's track record, multi-centre collaboration and free to read, so those were left out: it earned 23 of the 56 points we could judge it on, which is 41 out of 100.
| Component | Points | Why |
|---|---|---|
| Journal tier | 7 / 10 | Tier 3 — peer-reviewed journal (Angewandte Chemie (International ed. in English)) |
| Journal's citation record | — / 4 | No journal record from OpenAlex — left out of this score |
| Study design | 0 / 12 | No study design recorded — scores zero, not guessed (not stated in the record — scored 0) |
| Strength of evidence | 1 / 5 | Rated “low” in our summary |
| Peer-review validation | 10 / 10 | Peer-reviewed. Published as an article in a named peer-reviewed journal. |
| How recent | 3 / 5 | 2022 — within 6 year(s) of the newest study here |
| Sample size | 0 / 4 | Participant count not stated — scores zero (not stated in the record — scored 0) |
| Effect reported | 0 / 3 | No effect size reported — scores zero (not stated in the record — scored 0) |
| Studied in people | 0 / 3 | Not stated whether this was studied in people — scores zero (not stated in the record — scored 0) |
| Randomised / blinded | 0 / 2 | No randomisation or blinding stated — scores zero (not stated in the record — scored 0) |
| Times cited | — / 7 | No citation count (not found in OpenAlex) — left out of this score |
| Citations per year | — / 5 | No citation count or no year — left out of this score |
| Impact for its field and year | — / 6 | No field-weighted citation impact (OpenAlex has none yet) — left out of this score |
| Cited by studies here | — / 6 | Not yet checked against this library's citation graph — left out of this score |
| Still being cited | — / 4 | No yearly citation counts — left out of this score |
| Senior author's track record | — / 4 | Senior author's record not available — left out of this score |
| Institution's track record | — / 3 | No institution listed in OpenAlex — left out of this score |
| Multi-centre collaboration | — / 3 | Institutions not listed — left out of this score |
| Indexed in PubMed | 2 / 2 | Indexed in PubMed (PMID 35107860) |
| Free to read | — / 2 | Access not recorded — left out of this score |
| Points | 23 / 56 | Scored on 10 of 20 parts. We have no data for the rest, so they are left out: 23 of the 56 points we could judge it on is 41 out of 100. |
| Total | 41 / 100 | — |
What each of these 20 components measures, and what this score is not →
Read the original
Free copy unknown: no free-copy check has an answer for this record yet. That does not mean it is paywalled.
Related
Studies in the library that share something with this one. The line above each title says what.
Related studies in Prevention trials
Also tagged “proteostasis”
241 studies in the library carry the topic tag “proteostasis”; these are this study's neighbours in a fixed, alphabetical-by-hash tour of them, not the closest in content. All 241 tagged studies.