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In vitro analysis of mouse neural stem cells genetically modified to stably express human NGF by a novel multigenic viral expression system
Cenciarelli C, Budoni M, Mercanti D, Fernandez E, Pallini R, Aloe L, Cimino V, Maira G et al.. Neurological research, 2006-07-01
Topic: Brain proteins, microglia and lab models
Features of multipotentiality of engineered NSC-derived cell lines versus naive cells (control-NSC) were assessed by immunocytochemical analysis in differentiation conditions. Results Levels of secreted human NGF, from conditioned media obtained by rhNGF-NSC cultures, were found to be elevated in either proliferation or differentiation conditions as compared with control cells. Moreover, released hNGF demonstrated biologic activity on PC12 cells by a functional test of neurite outgrowth. Immunocytochemical analysis revealed that engineered NSC showed to be all positives for EGFP. After thirty passages in vitro in the presence of G418, engineered cells versus naive NSC cultures maintained their multipotentiality to differentiate into neurons, astrocytes and oligodendrocytes. The expression of neural differentiation markers, like microtubule associated protein 2 (MAP2) (a/b), glial fibrillary acidic protein (GFAP) and chondroitin sulphate proteoglycan (NG2), was not enhanced in rhNGF-NSC compared with control cells. Secreted hNGF increased axonal sprouting by rhNGF-NSC-derived neurons which was associated with ChAT expression. rhNGF-NSC may prospectively be good candidates for the treatment of either neurodegenerative diseases such as Alzheimer disease or central nervous system injuries.
Cite this study APA, Vancouver, BibTeX · download .bib or .ris
APA 7th
Cenciarelli, C., Budoni, M., Mercanti, D., Fernandez, E., Pallini, R., Aloe, L., et al. (2006). In vitro analysis of mouse neural stem cells genetically modified to stably express human NGF by a novel multigenic viral expression system. Neurological research. https://doi.org/10.1179/016164106x115161
Vancouver
Cenciarelli C, Budoni M, Mercanti D, Fernandez E, Pallini R, Aloe L, et al. In vitro analysis of mouse neural stem cells genetically modified to stably express human NGF by a novel multigenic viral expression system. Neurological research. 2006. doi:10.1179/016164106x115161. PMID: 16808880.
BibTeX
@article{cenciarelli2006-16808880,
author = {Cenciarelli, C. and Budoni, M. and Mercanti, D. and Fernandez, E. and Pallini, R. and Aloe, L. and Cimino, V. and Maira, G. and others},
title = {{In vitro analysis of mouse neural stem cells genetically modified to stably express human NGF by a novel multigenic viral expression system}},
journal = {Neurological research},
year = {2006},
doi = {10.1179/016164106x115161},
pmid = {16808880},
url = {https://doi.org/10.1179/016164106x115161}
}
Built from the record alzhub holds for this paper, not looked up. Volume, issue and page numbers are not held in the record. The record's author list ends in “et al.”, so not every author is named. Check the citation against the original before you use it.
Key findings
- Features of multipotentiality of engineered NSC-derived cell lines versus naive cells (control-NSC) were assessed by immunocytochemical analysis in differentiation conditions.
- Results Levels of secreted human NGF, from conditioned media obtained by rhNGF-NSC cultures, were found to be elevated in either proliferation or differentiation conditions as compared with control cells.
- Moreover, released hNGF demonstrated biologic activity on PC12 cells by a functional test of neurite outgrowth.
- Immunocytochemical analysis revealed that engineered NSC showed to be all positives for EGFP.
- After thirty passages in vitro in the presence of G418, engineered cells versus naive NSC cultures maintained their multipotentiality to differentiate into neurons, astrocytes and oligodendrocytes.
Why it matters
Mouse neural stem cells engineered to express human NGF released active NGF and increased axonal sprouting in vitro. This is early laboratory work, and the authors only suggest these cells may prospectively be candidates for treating neurodegenerative diseases such as Alzheimer's disease.
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Study details
- Evidence strengthLow
How this study scored 32/100
Published in a peer-reviewed journal. Scored zero for study design, sample size, effect reported and randomised / blinded because the record does not state it.
| Component | Points | Why |
|---|---|---|
| Journal tier | 7 / 10 | Tier 3 — peer-reviewed journal (Neurological research) |
| Journal's citation record | 1 / 4 | Neurological Research: journal h-index 108 (OpenAlex, 2026-10-29) |
| Study design | 0 / 12 | No study design recorded — scores zero, not guessed (not stated in the record — scored 0) |
| Strength of evidence | 1 / 5 | Rated “low” in our summary |
| Peer-review validation | 10 / 10 | Peer-reviewed. Published as an article in a named peer-reviewed journal. |
| How recent | 1 / 5 | 2006 — older than 20 years, still counted |
| Sample size | 0 / 4 | Participant count not stated — scores zero (not stated in the record — scored 0) |
| Effect reported | 0 / 3 | No effect size reported — scores zero (not stated in the record — scored 0) |
| Studied in people | 0 / 3 | Laboratory or animal study (title names “in vitro”) — scores zero |
| Randomised / blinded | 0 / 2 | No randomisation or blinding stated — scores zero (not stated in the record — scored 0) |
| Times cited | 3 / 7 | Cited 24 times (OpenAlex, 2026-10-10) |
| Citations per year | 1 / 5 | 1.1 citations a year over 22 year(s) since 2006 |
| Impact for its field and year | 2 / 6 | Cited 1.4× the average for papers of its field and year (OpenAlex FWCI) |
| Cited by studies here | 0 / 6 | No other study in this library cites it |
| Still being cited | 0 / 4 | Cited 1 times in 2024–2026 (2026 still in progress; OpenAlex) |
| Senior author's track record | 2 / 4 | Last-listed author Patrizia Casalbore: h-index 24 (OpenAlex, whole career) |
| Institution's track record | 1 / 3 | National Research Council: institution h-index 840 (OpenAlex) |
| Multi-centre collaboration | 1 / 3 | OpenAlex lists 3 institution(s) in 1 country |
| Indexed in PubMed | 2 / 2 | Indexed in PubMed (PMID 16808880) |
| Free to read | 0 / 2 | Behind a paywall — scores zero |
| Total | 32 / 100 | — |
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