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Developmental expression of a 56 kDa protein isolated from rat neocortex
Miller MW, Murtaugh M, Kuhn PE.. Brain research. Developmental brain research, 1994-09-01
Topic: Brain proteins, microglia and lab models
The expression of ALZ-50 immunoreactivity in the immature rat cortex was assessed using a variety of immunochemical techniques. The antigen recognized by ALZ-50 was isolated using immunoaffinity column chromatography. Unlike the ALZ-50-positive antigen found in the brains of people with Alzheimer's Disease (mol. wt. 68 kDa), the principal antigen expressed in the immature rat had a molecular weight of 56 kDa. The temporal expression of ALZ-50-positive antigens was determined with immunoblotting techniques. The expression of the 56 kDa protein appeared during the last prenatal week, increased to a peak during the second postnatal week and waned in the third postnatal week. Immunohistochemically, neonatal cortex contained many ALZ-50-positive neurons in the cortical subplate and the intermediate zone and a smattering of neurons in the cortical plate. The temporal expression of the ALZ-50-positive 56 kDa antigen was similar to that for fetal tau (tau-1); however, the principal protein identified by the anti-tau-1 antibody was considerably larger (being about 68-70 kDa). In contrast to ALZ-50, tau-1-immunoreactive elements were distributed in the subplate, the deep cortical plate, the intermediate zone and the subventricular zone. tau-1-positive axons passing through the superficial intermediate zone were conspicuous. Thus, the biochemical and anatomical evidence show that ALZ-50 and tau-1 recognize distinct proteins.
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APA 7th
Miller, M. W., Murtaugh, M., & Kuhn, P. E. (1994). Developmental expression of a 56 kDa protein isolated from rat neocortex. Brain research. Developmental brain research. https://doi.org/10.1016/0165-3806(94)90312-3
Vancouver
Miller MW, Murtaugh M, Kuhn PE. Developmental expression of a 56 kDa protein isolated from rat neocortex. Brain research. Developmental brain research. 1994. doi:10.1016/0165-3806(94)90312-3. PMID: 7813047.
BibTeX
@article{miller1994-7813047,
author = {Miller, M. W. and Murtaugh, M. and Kuhn, P. E.},
title = {{Developmental expression of a 56 kDa protein isolated from rat neocortex}},
journal = {Brain research. Developmental brain research},
year = {1994},
doi = {10.1016/0165-3806(94)90312-3},
pmid = {7813047},
url = {https://doi.org/10.1016/0165-3806(94)90312-3}
}
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Key findings
- The expression of the 56 kDa protein appeared during the last prenatal week, increased to a peak during the second postnatal week and waned in the third postnatal week.
- Immunohistochemically, neonatal cortex contained many ALZ-50-positive neurons in the cortical subplate and the intermediate zone and a smattering of neurons in the cortical plate.
- The temporal expression of the ALZ-50-positive 56 kDa antigen was similar to that for fetal tau (tau-1); however, the principal protein identified by the anti-tau-1 antibody was considerably larger (being about 68-70 kDa).
- In contrast to ALZ-50, tau-1-immunoreactive elements were distributed in the subplate, the deep cortical plate, the intermediate zone and the subventricular zone. tau-1-positive axons passing through the superficial intermediate zone were conspicuous.
- Thus, the biochemical and anatomical evidence show that ALZ-50 and tau-1 recognize distinct proteins.
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Study details
- Evidence strengthLow
How this study scored 36/100
Published in a peer-reviewed journal. Scored zero for study design, sample size, effect reported and randomised / blinded because the record does not state it. We have no data on journal's citation record, times cited, citations per year, impact for its field and year, cited by studies here, still being cited, senior author's track record, institution's track record and multi-centre collaboration, so those were left out: it earned 21 of the 58 points we could judge it on, which is 36 out of 100.
| Component | Points | Why |
|---|---|---|
| Journal tier | 7 / 10 | Tier 3 — peer-reviewed journal (Brain research. Developmental brain research) |
| Journal's citation record | — / 4 | No journal record from OpenAlex — left out of this score |
| Study design | 0 / 12 | No study design recorded — scores zero, not guessed (not stated in the record — scored 0) |
| Strength of evidence | 1 / 5 | Rated “low” in our summary |
| Peer-review validation | 10 / 10 | Peer-reviewed. Published as an article in a named peer-reviewed journal. |
| How recent | 1 / 5 | 1994 — older than 20 years, still counted |
| Sample size | 0 / 4 | Participant count not stated — scores zero (not stated in the record — scored 0) |
| Effect reported | 0 / 3 | No effect size reported — scores zero (not stated in the record — scored 0) |
| Studied in people | 0 / 3 | Laboratory or animal study (title names “rat”) — scores zero |
| Randomised / blinded | 0 / 2 | No randomisation or blinding stated — scores zero (not stated in the record — scored 0) |
| Times cited | — / 7 | No citation count (not found in OpenAlex) — left out of this score |
| Citations per year | — / 5 | No citation count or no year — left out of this score |
| Impact for its field and year | — / 6 | No field-weighted citation impact (OpenAlex has none yet) — left out of this score |
| Cited by studies here | — / 6 | Not yet checked against this library's citation graph — left out of this score |
| Still being cited | — / 4 | No yearly citation counts — left out of this score |
| Senior author's track record | — / 4 | Senior author's record not available — left out of this score |
| Institution's track record | — / 3 | No institution listed in OpenAlex — left out of this score |
| Multi-centre collaboration | — / 3 | Institutions not listed — left out of this score |
| Indexed in PubMed | 2 / 2 | Indexed in PubMed (PMID 7813047) |
| Free to read | 0 / 2 | Behind a paywall — scores zero |
| Points | 21 / 58 | Scored on 11 of 20 parts. We have no data for the rest, so they are left out: 21 of the 58 points we could judge it on is 36 out of 100. |
| Total | 36 / 100 | — |
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