Home › Research › Brain scans and imaging
Comparison of four staining methods on the detection of neuritic plaques
Wisniewski HM, Wen GY, Kim KS.. Acta neuropathologica, 1989-01-01
Topic: Amyloid, tau and blood-test diagnosis
The sensitivities of four staining methods for detecting the presence of neuritic plaques in the normal aging, Alzheimer disease (AD) and Down's syndrome (DS) brains were compared. The Bielschowsky method and immunostaining with monoclonal antibody (4G8, IgG2b) to beta-amyloid revealed the highest numbers of plaques. The thioflavin S-staining method showed about 38%-70% of plaques as shown by the Bielschowsky method. The Bodian method is the least sensitive method revealing about 26%-39% of plaques as compared with the Bielschowsky method. In the first part of this study, serial sections (6 microns) of the plaques from AD and DS brains were cut and stained with Bielschowsky, thioflavin S and Bodian methods, respectively. In the second part, Bielschowsky and immunostaining methods were used to stain serial sections (6 microns) of plaques from the same block of brain tissue. A Zeiss Axiophot fluorescence microscope, coupled with the Cambridge Quantiment 970 and Zeiss Videoplan computerized image analyzers, was used to quantify the number of plaques. Both Bielschowsky and immunostaining methods revealed the presence of both the peripheral zone (halo) and the central core of a classical plaque and resulted in the highest plaque counts. The central core of the thioflavin S-stained plaques were still clearly present, but the peripheral zone was barely visible. In Bodian stain, however, both central core and peripheral zone were poorly stained and not detectable by computer. They were quantified by human eye observation. The Bielschowsky method and immunostaining with pretreatment with formic acid are, therefore, the most sensitive methods showing the highest contrast of plaque image and thus facilitate the quantification of plaque by computer.(ABSTRACT TRUNCATED AT 250 WORDS)
Cite this study APA, Vancouver, BibTeX · download .bib or .ris
APA 7th
Wisniewski, H. M., Wen, G. Y., & Kim, K. S. (1989). Comparison of four staining methods on the detection of neuritic plaques. Acta neuropathologica. https://doi.org/10.1007/bf00687398
Vancouver
Wisniewski HM, Wen GY, Kim KS. Comparison of four staining methods on the detection of neuritic plaques. Acta neuropathologica. 1989. doi:10.1007/bf00687398. PMID: 2472039.
BibTeX
@article{wisniewski1989-2472039,
author = {Wisniewski, H. M. and Wen, G. Y. and Kim, K. S.},
title = {{Comparison of four staining methods on the detection of neuritic plaques}},
journal = {Acta neuropathologica},
year = {1989},
doi = {10.1007/bf00687398},
pmid = {2472039},
url = {https://doi.org/10.1007/bf00687398}
}
Built from the record alzhub holds for this paper, not looked up. Volume, issue and page numbers are not held in the record. Check the citation against the original before you use it.
Key findings
- The Bielschowsky method and immunostaining with monoclonal antibody (4G8, IgG2b) to beta-amyloid revealed the highest numbers of plaques.
- The Bodian method is the least sensitive method revealing about 26%-39% of plaques as compared with the Bielschowsky method.
- Both Bielschowsky and immunostaining methods revealed the presence of both the peripheral zone (halo) and the central core of a classical plaque and resulted in the highest plaque counts.
New to this subject? Start with our plain-English guide: Brain scans & tests →
Study details
- Evidence strengthLow
How this study scored 40/100
Published in a leading specialty journal. Scored zero for study design, sample size, effect reported, studied in people and randomised / blinded because the record does not state it. We have no data on journal's citation record, times cited, citations per year, impact for its field and year, cited by studies here, still being cited, senior author's track record, institution's track record and multi-centre collaboration, so those were left out: it earned 23 of the 58 points we could judge it on, which is 40 out of 100.
| Component | Points | Why |
|---|---|---|
| Journal tier | 9 / 10 | Tier 2 — leading specialty journal (Acta neuropathologica) |
| Journal's citation record | — / 4 | No journal record from OpenAlex — left out of this score |
| Study design | 0 / 12 | No study design recorded — scores zero, not guessed (not stated in the record — scored 0) |
| Strength of evidence | 1 / 5 | Rated “low” in our summary |
| Peer-review validation | 10 / 10 | Peer-reviewed. Published as an article in a named peer-reviewed journal. |
| How recent | 1 / 5 | 1989 — older than 20 years, still counted |
| Sample size | 0 / 4 | Participant count not stated — scores zero (not stated in the record — scored 0) |
| Effect reported | 0 / 3 | No effect size reported — scores zero (not stated in the record — scored 0) |
| Studied in people | 0 / 3 | Not stated whether this was studied in people — scores zero (not stated in the record — scored 0) |
| Randomised / blinded | 0 / 2 | No randomisation or blinding stated — scores zero (not stated in the record — scored 0) |
| Times cited | — / 7 | No citation count (not found in OpenAlex) — left out of this score |
| Citations per year | — / 5 | No citation count or no year — left out of this score |
| Impact for its field and year | — / 6 | No field-weighted citation impact (OpenAlex has none yet) — left out of this score |
| Cited by studies here | — / 6 | Not yet checked against this library's citation graph — left out of this score |
| Still being cited | — / 4 | No yearly citation counts — left out of this score |
| Senior author's track record | — / 4 | Senior author's record not available — left out of this score |
| Institution's track record | — / 3 | No institution listed in OpenAlex — left out of this score |
| Multi-centre collaboration | — / 3 | Institutions not listed — left out of this score |
| Indexed in PubMed | 2 / 2 | Indexed in PubMed (PMID 2472039) |
| Free to read | 0 / 2 | Behind a paywall — scores zero |
| Points | 23 / 58 | Scored on 11 of 20 parts. We have no data for the rest, so they are left out: 23 of the 58 points we could judge it on is 40 out of 100. |
| Total | 40 / 100 | — |
What each of these 20 components measures, and what this score is not →
Read the original
Free copy unknown: no free-copy check has an answer for this record yet. That does not mean it is paywalled.
Related
Studies in the library that share something with this one. The line above each title says what.
Related studies in Brain scans and imaging
Also tagged “four”
3 studies in the library carry the topic tag “four”; these are this study's neighbours in a fixed, alphabetical-by-hash tour of them, not the closest in content. All 3 tagged studies.