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Radionuclide Selection Influences Imaging Outcomes in Immuno-PET with a Brain-Penetrating Anti–Amyloid-β Antibody
Sara Lopes van den Broek, Klas Bratteby, Ximena Aguilar, Thuy Tran, Stina Syvänen, Dag Sehlin. Journal of Nuclear Medicine, 2025-12-11
Topic: Amyloid, tau and blood-test diagnosis
PET imaging demonstrated high cortical brain uptake of all 3 radiotracers in Tg-ArcSwe mice. Labeling with the metals 89Zr and 64Cu produced the highest overall brain signal in both Tg-ArcSwe and WT mice. [89Zr]Zr-DFO*-Lec-Fab8D3 and [124I]I-Lec-Fab8D3 demonstrated the greatest discrimination between Tg-ArcSwe and WT mice, with [124I]I-Lec-Fab8D3 exhibiting the most pronounced regional differences. Ex vivo analyses corroborated the PET findings, and immunostaining confirmed radiotracer colocalization with Aβ deposits. Immuno-PET imaging with radiolabeled Lec-Fab8D3 enables specific detection of brain Aβ pathology. Because of its residualizing properties, 89Zr produced the highest overall signal, whereas 124I yielded greater regional contrast, despite lower total brain signal. These findings enhance our understanding of the intrabrain distribution of bispecific antibodies and highlight the importance of radionuclide selection and its impact on immuno-PET outcomes.
Cite this study APA, Vancouver, BibTeX · download .bib or .ris
APA 7th
van den Broek, S. L., Bratteby, K., Aguilar, X., Tran, T., Syvänen, S., & Sehlin, D. (2025). Radionuclide Selection Influences Imaging Outcomes in Immuno-PET with a Brain-Penetrating Anti–Amyloid-β Antibody. Journal of Nuclear Medicine. https://doi.org/10.2967/jnumed.125.271194
Vancouver
van den Broek SL, Bratteby K, Aguilar X, Tran T, Syvänen S, Sehlin D. Radionuclide Selection Influences Imaging Outcomes in Immuno-PET with a Brain-Penetrating Anti–Amyloid-β Antibody. Journal of Nuclear Medicine. 2025. doi:10.2967/jnumed.125.271194. PMID: 41381239.
BibTeX
@article{vandenbroek2025-41381239,
author = {van den Broek, Sara Lopes and Bratteby, Klas and Aguilar, Ximena and Tran, Thuy and Syvänen, Stina and Sehlin, Dag},
title = {{Radionuclide Selection Influences Imaging Outcomes in Immuno-PET with a Brain-Penetrating Anti–Amyloid-β Antibody}},
journal = {Journal of Nuclear Medicine},
year = {2025},
doi = {10.2967/jnumed.125.271194},
pmid = {41381239},
url = {https://doi.org/10.2967/jnumed.125.271194}
}
Built from the record alzhub holds for this paper, not looked up. Volume, issue and page numbers are not held in the record. Check the citation against the original before you use it.
Key findings
- PET imaging demonstrated high cortical brain uptake of all 3 radiotracers in Tg-ArcSwe mice.
- Labeling with the metals 89Zr and 64Cu produced the highest overall brain signal in both Tg-ArcSwe and WT mice. [89Zr]Zr-DFO*-Lec-Fab8D3 and [124I]I-Lec-Fab8D3 demonstrated the greatest discrimination between Tg-ArcSwe and WT mice, with [124I]I-Lec-Fab8D3 exhibiting the most pronounced regional differences.
- Ex vivo analyses corroborated the PET findings, and immunostaining confirmed radiotracer colocalization with Aβ deposits.
- Immuno-PET imaging with radiolabeled Lec-Fab8D3 enables specific detection of brain Aβ pathology.
- Because of its residualizing properties, 89Zr produced the highest overall signal, whereas 124I yielded greater regional contrast, despite lower total brain signal.
Why it matters
In Tg-ArcSwe mice, immuno-PET with radiolabeled Lec-Fab8D3 enabled specific detection of brain Aβ pathology, with 89Zr producing the highest overall signal and 124I yielding greater regional contrast. These findings are from animal studies and concern imaging of amyloid, not treatment, cure or prevention of Alzheimer's disease.
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Study details
- Evidence strengthLow
How this study scored 32/100
Published in a peer-reviewed journal. Scored zero for study design, sample size, effect reported, studied in people and randomised / blinded because the record does not state it.
| Component | Points | Why |
|---|---|---|
| Journal tier | 7 / 10 | Tier 3 — peer-reviewed journal (Journal of Nuclear Medicine) |
| Journal's citation record | 2 / 4 | Journal of Nuclear Medicine: journal h-index 230 (OpenAlex, 2026-10-29) |
| Study design | 0 / 12 | No study design recorded — scores zero, not guessed (not stated in the record — scored 0) |
| Strength of evidence | 1 / 5 | Rated “low” in our summary |
| Peer-review validation | 10 / 10 | Peer-reviewed. Published as an article in a named peer-reviewed journal. |
| How recent | 4 / 5 | 2025 — within 3 year(s) of the newest study here |
| Sample size | 0 / 4 | Participant count not stated — scores zero (not stated in the record — scored 0) |
| Effect reported | 0 / 3 | No effect size reported — scores zero (not stated in the record — scored 0) |
| Studied in people | 0 / 3 | Not stated whether this was studied in people — scores zero (not stated in the record — scored 0) |
| Randomised / blinded | 0 / 2 | No randomisation or blinding stated — scores zero (not stated in the record — scored 0) |
| Times cited | 0 / 7 | Cited 0 times (OpenAlex, 2026-10-10) |
| Citations per year | 0 / 5 | 0.0 citations a year over 3 year(s) since 2025 |
| Impact for its field and year | 0 / 6 | Cited 0.0× the average for papers of its field and year (OpenAlex FWCI) |
| Cited by studies here | 0 / 6 | No other study in this library cites it |
| Still being cited | 0 / 4 | Cited 0 times in 2024–2026 (2026 still in progress; OpenAlex) |
| Senior author's track record | 2 / 4 | Last-listed author Dag Sehlin: h-index 38 (OpenAlex, whole career) |
| Institution's track record | 1 / 3 | Karolinska Institutet: institution h-index 1,477 (OpenAlex) |
| Multi-centre collaboration | 1 / 3 | OpenAlex lists 3 institution(s) in 1 country |
| Indexed in PubMed | 2 / 2 | Indexed in PubMed (PMID 41381239) |
| Free to read | 2 / 2 | Free to read |
| Total | 32 / 100 | — |
What each of these 20 components measures, and what this score is not →
Read the original
- DOI: 10.2967/jnumed.125.271194
- PubMed: 41381239
- Original source →
- Free full text: Journal of Nuclear Medicine →
Full text: free to read — OpenAlex lists this copy as free to read: the link above (checked 6 Feb 1020).
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