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Portable platform for measuring amyloid beta 42/40 ratio via photooxidation-induced fluorescence amplification
Sanghag Ko, Hyunjun Bae, Daewon Kim, Yeonju Lee, Isaac Choi, Dain Lee, Dong Hwan Choi, Hyung Chul Kim et al.. Lab on a chip, 2025-08-05
Topic: Plasma and spinal-fluid biomarkers
Alzheimer's disease (AD) is a serious health condition that exacerbates with age. Among various AD biomarkers, the measurement of the Aβ 42/40 ratio (i.e., ratio of Aβ 42 and Aβ 40 concentrations) has garnered prominence for the early identification of AD patients and the development of disease-modifying treatments. Approaches such as positron emission tomography examinations and biomarker measurements in cerebrospinal fluid and blood plasma face constraints related to expense and procedural complexity. To address these issues, we developed and evaluated the efficacy of a portable platform for detecting amyloid beta (Aβ) 40 and 42 in plasma, utilizing photooxidation-induced fluorescence amplification (PIFA). We conducted a comparative analysis of Aβ 42/40 measurements between the PIFA and single-molecule immunoassay (SiMoA) platforms. By measuring 38 cases of subjective cognitive decline (SCD), 24 cases of amnestic mild cognitive impairment (aMCI), and 46 cases of AD dementia samples, we observed a significant difference in Aβ 42/40 ratios between the SCD and aMCI groups. The PIFA platform demonstrated an area under the curve compared to that of the SiMoA platform, which is currently the most precise method for Aβ 42/40 ratio measurement. Consequently, the PIFA platform presents a viable cost-effective tool for detecting the Aβ 42/40 ratio.
Key findings
- By measuring 38 cases of subjective cognitive decline (SCD), 24 cases of amnestic mild cognitive impairment (aMCI), and 46 cases of AD dementia samples, we observed a significant difference in Aβ 42/40 ratios between the SCD and aMCI groups.
- The PIFA platform demonstrated an area under the curve compared to that of the SiMoA platform, which is currently the most precise method for Aβ 42/40 ratio measurement.
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Study details
- Evidence strengthLow
How this study scored 43/100
Published in a peer-reviewed journal. Scored zero for study design, sample size, effect reported, studied in people and randomised / blinded because the record does not state it. We have no data on journal's citation record, times cited, citations per year, impact for its field and year, cited by studies here, still being cited, senior author's track record, institution's track record, multi-centre collaboration and free to read, so those were left out: it earned 24 of the 56 points we could judge it on, which is 43 out of 100.
| Component | Points | Why |
|---|---|---|
| Journal tier | 7 / 10 | Tier 3 — peer-reviewed journal (Lab on a chip) |
| Journal's citation record | — / 4 | No journal record from OpenAlex — left out of this score |
| Study design | 0 / 12 | No study design recorded — scores zero, not guessed (not stated in the record — scored 0) |
| Strength of evidence | 1 / 5 | Rated “low” in our summary |
| Peer-review validation | 10 / 10 | Peer-reviewed. Published as an article in a named peer-reviewed journal. |
| How recent | 4 / 5 | 2025 — within 3 year(s) of the newest study here |
| Sample size | 0 / 4 | Participant count not stated — scores zero (not stated in the record — scored 0) |
| Effect reported | 0 / 3 | No effect size reported — scores zero (not stated in the record — scored 0) |
| Studied in people | 0 / 3 | Not stated whether this was studied in people — scores zero (not stated in the record — scored 0) |
| Randomised / blinded | 0 / 2 | No randomisation or blinding stated — scores zero (not stated in the record — scored 0) |
| Times cited | — / 7 | No citation count (not found in OpenAlex) — left out of this score |
| Citations per year | — / 5 | No citation count or no year — left out of this score |
| Impact for its field and year | — / 6 | No field-weighted citation impact (OpenAlex has none yet) — left out of this score |
| Cited by studies here | — / 6 | Not yet checked against this library's citation graph — left out of this score |
| Still being cited | — / 4 | No yearly citation counts — left out of this score |
| Senior author's track record | — / 4 | Senior author's record not available — left out of this score |
| Institution's track record | — / 3 | No institution listed in OpenAlex — left out of this score |
| Multi-centre collaboration | — / 3 | Institutions not listed — left out of this score |
| Indexed in PubMed | 2 / 2 | Indexed in PubMed (PMID 40546070) |
| Free to read | — / 2 | Access not recorded — left out of this score |
| Points | 24 / 56 | Scored on 10 of 20 parts. We have no data for the rest, so they are left out: 24 of the 56 points we could judge it on is 43 out of 100. |
| Total | 43 / 100 | — |
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Cite this study APA, Vancouver, BibTeX · download .bib or .ris
Built from the record alzhub holds for this paper, not looked up. Volume, issue and page numbers are not held in the record. The record's author list ends in “et al.”, so not every author is named. Check the citation against the original before you use it.
APA 7th
Ko, S., Bae, H., Kim, D., Lee, Y., Choi, I., Lee, D., et al. (2025). Portable platform for measuring amyloid beta 42/40 ratio via photooxidation-induced fluorescence amplification. Lab on a chip. https://doi.org/10.1039/d5lc00235d
Vancouver
Ko S, Bae H, Kim D, Lee Y, Choi I, Lee D, et al. Portable platform for measuring amyloid beta 42/40 ratio via photooxidation-induced fluorescence amplification. Lab on a chip. 2025. doi:10.1039/d5lc00235d. PMID: 40546070.
BibTeX
@article{ko2025-40546070,
author = {Ko, Sanghag and Bae, Hyunjun and Kim, Daewon and Lee, Yeonju and Choi, Isaac and Lee, Dain and Choi, Dong Hwan and Kim, Hyung Chul and others},
title = {{Portable platform for measuring amyloid beta 42/40 ratio via photooxidation-induced fluorescence amplification}},
journal = {Lab on a chip},
year = {2025},
doi = {10.1039/d5lc00235d},
pmid = {40546070},
url = {https://doi.org/10.1039/d5lc00235d}
}
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