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41/100

✓ Peer-reviewed alzhub usefulness score 41 out of 100. Risk genes beyond APOE

Topic: Brain proteins, microglia and lab models

ABSTRACT Alzheimer's disease (AD), one of the most common types of dementia, is an urgent and growing global challenge. AD pathogenesis is associated with increased activity of the acetylcholinesterase enzyme (AChE) and the β ‐site amyloid precursor protein cleaving enzyme ( β ‐secretase, BACE1). This study aimed to evaluate the AChE and BACE1 inhibitory activities of the n ‐hexane soluble fraction of Crateva magna leaf extract (CMHF) and its cytotoxic properties against cancer and normal cell lines using MTT assay, also this study aimed to identify the volatile constituents of CMHF by gas chromatography‐mass spectrometry (GC‐MS) analysis. GC‐MS analysis revealed a total of 13 metabolites which represent 92.42% of the detected compounds. Phytol was the major constituent of CMHF, representing 20.52% followed by ( Z, Z, Z )‐9,12,15‐octadecatrienoic acid ethyl ester (19.04%), γ ‐sitosterol (13.71%), hexadecanoic acid ethyl ester (12.63%) and others. CMHF revealed potent AChE and BACE1 inhibitory activities with IC 50 = 0.114 ± 0.006 and 1.723 ± 0.1 µg/mL, respectively. In conclusion, these findings indicate that CMHF may possess anti‐Alzheimer efficacy. Furthermore, the absence of cytotoxicity against HepG‐2, HCT‐116, MCF‐7, and WISH cell lines reinforces its safety profile. Therefore, CMHF could be considered as a natural and safe alternative for managing AD.

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APA 7th

Talaat, A. N., Ibrahim, N., Ayoub, I. M., Labib, R. M., & Singab, A. N. B. (2025). Gas Chromatography‐Mass Spectrometry Profiling of Crateva magna Leaf Lipophilic Extract and Evaluation of Its Anti‐Alzheimer and Cytotoxic Properties. Chemistry & Biodiversity. https://doi.org/10.1002/cbdv.202402770

Vancouver

Talaat AN, Ibrahim N, Ayoub IM, Labib RM, Singab ANB. Gas Chromatography‐Mass Spectrometry Profiling of Crateva magna Leaf Lipophilic Extract and Evaluation of Its Anti‐Alzheimer and Cytotoxic Properties. Chemistry & Biodiversity. 2025. doi:10.1002/cbdv.202402770. PMID: 39966086.

BibTeX

@article{talaat2025-39966086,
  author  = {Talaat, Aya N. and Ibrahim, Nehal and Ayoub, Iriny M. and Labib, Rola Milad and Singab, Abdel Nasser B.},
  title   = {{Gas Chromatography‐Mass Spectrometry Profiling of Crateva magna Leaf Lipophilic Extract and Evaluation of Its Anti‐Alzheimer and Cytotoxic Properties}},
  journal = {Chemistry \& Biodiversity},
  year    = {2025},
  doi     = {10.1002/cbdv.202402770},
  pmid    = {39966086},
  url     = {https://doi.org/10.1002/cbdv.202402770}
}

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Key findings

AD pathogenesis is associated with increased activity of the acetylcholinesterase enzyme (AChE) and the β ‐site amyloid precursor protein cleaving enzyme ( β ‐secretase, BACE1).
GC‐MS analysis revealed a total of 13 metabolites which represent 92.42% of the detected compounds.
CMHF revealed potent AChE and BACE1 inhibitory activities with IC 50 = 0.114 ± 0.006 and 1.723 ± 0.1 µg/mL, respectively.
In conclusion, these findings indicate that CMHF may possess anti‐Alzheimer efficacy.

Why it matters

The n-hexane fraction of Crateva magna leaf extract showed AChE and BACE1 inhibitory activity in the laboratory and no cytotoxicity against several cell lines. The authors suggest it may possess anti-Alzheimer efficacy, but this is an in-vitro study and no testing in animals or people was reported.

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Study details

Participants13
Effect size92.42%; 20.52%; 19.04%
Evidence strengthLow

How this study scored 41/100

Published in a peer-reviewed journal. Scored zero for study design and randomized / blinded because the record does not state it.

Component	Points	Why
Journal tier	7 / 10	Tier 3 — peer-reviewed journal (Chemistry & Biodiversity)
Journal's citation record	1 / 4	Chemistry & Biodiversity: journal h-index 99 (OpenAlex, 2026-10-29)
Study design	0 / 12	No study design recorded — scores zero, not guessed (not stated in the record — scored 0)
Strength of evidence	1 / 5	Rated “low” in our summary
Peer-review validation	10 / 10	Peer-reviewed. Published as an article in a named peer-reviewed journal.
How recent	4 / 5	2025 — within 3 year(s) of the newest study here
Sample size	1 / 4	13 participants
Effect reported	2 / 3	Effect reported as a number, without an interval or test
Studied in people	3 / 3	Studied in people (participant count stated)
Randomized / blinded	0 / 2	No randomization or blinding stated — scores zero (not stated in the record — scored 0)
Times cited	1 / 7	Cited 3 times (OpenAlex, 2026-10-10)
Citations per year	1 / 5	1.0 citations a year over 3 year(s) since 2025
Impact for its field and year	3 / 6	Cited 3.2× the average for papers of its field and year (OpenAlex FWCI)
Cited by studies here	0 / 6	No other study in this library cites it
Still being cited	1 / 4	Cited 3 times in 2024–2026 (2026 still in progress; OpenAlex)
Senior author's track record	2 / 4	Last-listed author Abdel Nasser B. Singab: h-index 45 (OpenAlex, whole career)
Institution's track record	0 / 3	Ain Shams University: institution h-index 314 (OpenAlex)
Multicenter collaboration	0 / 3	OpenAlex lists 1 institution(s) in 1 country — scores zero (affiliation lists can be incomplete)
Indexed in PubMed	2 / 2	Indexed in PubMed (PMID 39966086)
Free to read	2 / 2	Free to read
Total	41 / 100	—

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