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Effect of stress and peripheral immune activation on astrocyte activation in transgenic bioluminescent Gfap‐luc mice
Steven Biesmans, Paul D. Acton, Carlos Cotto, Xavier Langlois, Luc Ver Donck, Jan A. Bouwknecht, Sarah‐Ann Aelvoet, Niels Hellings et al.. Glia, 2015-02-17
Topic: Inflammation, microglia and astrocytes
Neuroinflammation and the accompanying activation of glial cells is an important feature of many neurodegenerative conditions. It is known that factors such as peripheral infections and stress can influence immune processes in the brain. However, the effect of these stressors on astrocyte activation in vivo remains elusive. In this study, transgenic Gfap‐luc mice expressing the luciferase gene under the transcriptional control of the glial fibrillary acidic protein promoter were used to quantify the kinetics of in vivo astrocyte activation following immune challenges relevant to clinical inflammation. It was found that astrocytes respond rapidly to peripheral immune activation elicited by either bacterial lipopolysaccharide (LPS) or the viral mimetic polyinosinic:polycytidylic acid (poly(I:C)). By measuring bioluminescence and 18‐kDa translocator protein radioligand binding in the same animal it was observed that LPS induces both astrocyte as well as microglial activation at 6 h post‐administration. Furthermore, the astrocyte response decreased upon repeated systemic LPS injections, indicating development of tolerance to the LPS challenge. Finally, restraining Gfap‐luc mice for 1 h daily on 5 consecutive days did not affect brain bioluminescence, thereby indicating that sub‐chronic stress does not influence astrocyte activation under unchallenged conditions. However, stressed animals showed a reduced response to a subsequent systemic LPS injection, suggesting that the immune system is compromised in these animals. Here, we demonstrate that Gfap‐luc mice can be used to study astrocyte activation in response to stimuli relevant for clinical inflammation and that this approach may provide a more complete characterization of existing and novel models of neuroinflammation. GLIA 2015;63:1126–1137
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APA 7th
Biesmans, S., Acton, P. D., Cotto, C., Langlois, X., Donck, L. V., Bouwknecht, J. A., et al. (2015). Effect of stress and peripheral immune activation on astrocyte activation in transgenic bioluminescent Gfap‐luc mice. Glia. https://doi.org/10.1002/glia.22804
Vancouver
Biesmans S, Acton PD, Cotto C, Langlois X, Donck LV, Bouwknecht JA, et al. Effect of stress and peripheral immune activation on astrocyte activation in transgenic bioluminescent Gfap‐luc mice. Glia. 2015. doi:10.1002/glia.22804.
BibTeX
@article{biesmans2015-5f2cd130,
author = {Biesmans, Steven and Acton, Paul D. and Cotto, Carlos and Langlois, Xavier and Donck, Luc Ver and Bouwknecht, Jan A. and Aelvoet, Sarah‐Ann and Hellings, Niels and others},
title = {{Effect of stress and peripheral immune activation on astrocyte activation in transgenic bioluminescent Gfap‐luc mice}},
journal = {Glia},
year = {2015},
doi = {10.1002/glia.22804},
url = {https://doi.org/10.1002/glia.22804}
}
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Key findings
- However, stressed animals showed a reduced response to a subsequent systemic LPS injection, suggesting that the immune system is compromised in these animals.
Study details
- Evidence strengthLow
How this study scored 36/100
Published in a peer-reviewed journal. Scored zero for study design, sample size, effect reported and randomised / blinded because the record does not state it. We have no data on journal's citation record, times cited, citations per year, impact for its field and year, cited by studies here, still being cited, senior author's track record, institution's track record, multi-centre collaboration and free to read, so those were left out: it earned 20 of the 56 points we could judge it on, which is 36 out of 100.
| Component | Points | Why |
|---|---|---|
| Journal tier | 7 / 10 | Tier 3 — peer-reviewed journal (Glia) |
| Journal's citation record | — / 4 | No journal record from OpenAlex — left out of this score |
| Study design | 0 / 12 | No study design recorded — scores zero, not guessed (not stated in the record — scored 0) |
| Strength of evidence | 1 / 5 | Rated “low” in our summary |
| Peer-review validation | 10 / 10 | Peer-reviewed. Published as an article in a named peer-reviewed journal. |
| How recent | 1 / 5 | 2015 — within 20 year(s) of the newest study here |
| Sample size | 0 / 4 | Participant count not stated — scores zero (not stated in the record — scored 0) |
| Effect reported | 0 / 3 | No effect size reported — scores zero (not stated in the record — scored 0) |
| Studied in people | 0 / 3 | Laboratory or animal study (title names “transgenic”) — scores zero |
| Randomised / blinded | 0 / 2 | No randomisation or blinding stated — scores zero (not stated in the record — scored 0) |
| Times cited | — / 7 | No citation count (not found in OpenAlex) — left out of this score |
| Citations per year | — / 5 | No citation count or no year — left out of this score |
| Impact for its field and year | — / 6 | No field-weighted citation impact (OpenAlex has none yet) — left out of this score |
| Cited by studies here | — / 6 | Not yet checked against this library's citation graph — left out of this score |
| Still being cited | — / 4 | No yearly citation counts — left out of this score |
| Senior author's track record | — / 4 | Senior author's record not available — left out of this score |
| Institution's track record | — / 3 | No institution listed in OpenAlex — left out of this score |
| Multi-centre collaboration | — / 3 | Institutions not listed — left out of this score |
| Indexed in PubMed | 1 / 2 | Not in PubMed, but has a DOI |
| Free to read | — / 2 | Access not recorded — left out of this score |
| Points | 20 / 56 | Scored on 10 of 20 parts. We have no data for the rest, so they are left out: 20 of the 56 points we could judge it on is 36 out of 100. |
| Total | 36 / 100 | — |
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