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A transistor-based label-free immunosensor for rapid detection of tau protein
García-Chamé MÁ, Gutiérrez-Sanz Ó, Ercan-Herbst E, Haustein N, Filipiak MS, Ehrnhöfer DE, Tarasov A.. Biosensors & bioelectronics, 2020-03-04
Topic: Brain proteins, microglia and lab models
Tau protein in cerebrospinal fluid (CSF) is a central and relevant biomarker of Alzheimer's disease (AD) that correlates with the severity of dementia. Unfortunately, so far, direct label-free detection of tau remains a challenge. Here, we present a transistor-based biosensor that detects the net charge of tau protein directly under physiological conditions. To achieve this, readily available whole anti-tau IgG antibodies are co-immobilized on the sensor surface with polyethylene glycol (PEG) molecules of different molecular weight. We show that by increasing the PEG size from 10 kDa to 20 kDa, the electrical response upon binding of tau improves significantly. These results support recent theoretical work that predicted larger PEGs to form a thicker surface layer with a higher detectable analyte charge. With 20 kDa PEG, we demonstrate label-free tau detection in a wide concentration range with detection limits <1 pM in 150 mM buffer and cell culture media, as well as < 10 pM in artificial CSF. This purely electrical method allows fast and simple tau detection within 30 min without sample processing, washing steps, or labeled detection antibodies. By exchanging the capture antibody, the platform is also amenable to different biomarkers and may enable future diagnostic tools for AD and other diseases.
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APA 7th
MÁ, G.-C., Ó, G.-S., Ercan-Herbst, E., Haustein, N., Filipiak, M. S., Ehrnhöfer, D. E., et al. (2020). A transistor-based label-free immunosensor for rapid detection of tau protein. Biosensors & bioelectronics. https://doi.org/10.1016/j.bios.2020.112129
Vancouver
MÁ G.-C, Ó G.-S, Ercan-Herbst E, Haustein N, Filipiak MS, Ehrnhöfer DE, et al. A transistor-based label-free immunosensor for rapid detection of tau protein. Biosensors & bioelectronics. 2020. doi:10.1016/j.bios.2020.112129. PMID: 32364931.
BibTeX
@article{ma2020-32364931,
author = {MÁ, García-Chamé and Ó, Gutiérrez-Sanz and Ercan-Herbst, E. and Haustein, N. and Filipiak, M. S. and Ehrnhöfer, D. E. and Tarasov, A.},
title = {{A transistor-based label-free immunosensor for rapid detection of tau protein}},
journal = {Biosensors \& bioelectronics},
year = {2020},
doi = {10.1016/j.bios.2020.112129},
pmid = {32364931},
url = {https://doi.org/10.1016/j.bios.2020.112129}
}
Built from the record alzhub holds for this paper, not looked up. Volume, issue and page numbers are not held in the record. The record names 7 authors; APA and Vancouver show the first six, BibTeX and the downloads carry all of them. Check the citation against the original before you use it.
Key findings
- We show that by increasing the PEG size from 10 kDa to 20 kDa, the electrical response upon binding of tau improves significantly.
- These results support recent theoretical work that predicted larger PEGs to form a thicker surface layer with a higher detectable analyte charge.
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Study details
- Evidence strengthLow
How this study scored 38/100
Published in a peer-reviewed journal. Scored zero for study design, sample size, effect reported, studied in people and randomised / blinded because the record does not state it. We have no data on journal's citation record, times cited, citations per year, impact for its field and year, cited by studies here, still being cited, senior author's track record, institution's track record and multi-centre collaboration, so those were left out: it earned 22 of the 58 points we could judge it on, which is 38 out of 100.
| Component | Points | Why |
|---|---|---|
| Journal tier | 7 / 10 | Tier 3 — peer-reviewed journal (Biosensors & bioelectronics) |
| Journal's citation record | — / 4 | No journal record from OpenAlex — left out of this score |
| Study design | 0 / 12 | No study design recorded — scores zero, not guessed (not stated in the record — scored 0) |
| Strength of evidence | 1 / 5 | Rated “low” in our summary |
| Peer-review validation | 10 / 10 | Peer-reviewed. Published as an article in a named peer-reviewed journal. |
| How recent | 2 / 5 | 2020 — within 10 year(s) of the newest study here |
| Sample size | 0 / 4 | Participant count not stated — scores zero (not stated in the record — scored 0) |
| Effect reported | 0 / 3 | No effect size reported — scores zero (not stated in the record — scored 0) |
| Studied in people | 0 / 3 | Not stated whether this was studied in people — scores zero (not stated in the record — scored 0) |
| Randomised / blinded | 0 / 2 | No randomisation or blinding stated — scores zero (not stated in the record — scored 0) |
| Times cited | — / 7 | No citation count (not found in OpenAlex) — left out of this score |
| Citations per year | — / 5 | No citation count or no year — left out of this score |
| Impact for its field and year | — / 6 | No field-weighted citation impact (OpenAlex has none yet) — left out of this score |
| Cited by studies here | — / 6 | Not yet checked against this library's citation graph — left out of this score |
| Still being cited | — / 4 | No yearly citation counts — left out of this score |
| Senior author's track record | — / 4 | Senior author's record not available — left out of this score |
| Institution's track record | — / 3 | No institution listed in OpenAlex — left out of this score |
| Multi-centre collaboration | — / 3 | Institutions not listed — left out of this score |
| Indexed in PubMed | 2 / 2 | Indexed in PubMed (PMID 32364931) |
| Free to read | 0 / 2 | Behind a paywall — scores zero |
| Points | 22 / 58 | Scored on 11 of 20 parts. We have no data for the rest, so they are left out: 22 of the 58 points we could judge it on is 38 out of 100. |
| Total | 38 / 100 | — |
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