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A technique for serial collection of cerebrospinal fluid from the cisterna magna in mouse
Liu L, Duff K.. Journal of visualized experiments : JoVE, 2008-11-10
Topic: Brain proteins, microglia and lab models
Alzheimer's disease (AD) is a progressive neurodegenerative disease that is pathologically characterized by extracellular deposition of beta-amyloid peptide (Abeta) and intraneuronal accumulation of hyperphosphorylated tau protein. Because cerebrospinal fluid (CSF) is in direct contact with the extracellular space of the brain, it provides a reflection of the biochemical changes in the brain in response to pathological processes. CSF from AD patients shows a decrease in the 42 amino-acid form of Abeta (Abeta42), and increases in total tau and hyperphosphorylated tau, though the mechanisms responsible for these changes are still not fully understood. Transgenic (Tg) mouse models of AD provide an excellent opportunity to investigate how and why Abeta or tau levels in CSF change as the disease progresses. Here, we demonstrate a refined cisterna magna puncture technique for CSF sampling from the mouse. This extremely gentle sampling technique allows serial CSF samples to be obtained from the same mouse at 2-3 month intervals which greatly minimizes the confounding effect of between-mouse variability in Abeta or tau levels, making it possible to detect subtle alterations over time. In combination with Abeta and tau ELISA, this technique will be useful for studies designed to investigate the relationship between the levels of CSF Abeta42 and tau, and their metabolism in the brain in AD mouse models. Studies in Tg mice could provide important validation as to the potential of CSF Abeta or tau levels to be used as biological markers for monitoring disease progression, and to monitor the effect of therapeutic interventions. As the mice can be sacrificed and the brains can be examined for biochemical or histological changes, the mechanisms underlying the CSF changes can be better assessed. These data are likely to be informative for interpretation of human AD CSF changes.
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APA 7th
Liu, L., & Duff, K. (2008). A technique for serial collection of cerebrospinal fluid from the cisterna magna in mouse. Journal of visualized experiments : JoVE. https://doi.org/10.3791/960
Vancouver
Liu L, Duff K. A technique for serial collection of cerebrospinal fluid from the cisterna magna in mouse. Journal of visualized experiments : JoVE. 2008. doi:10.3791/960. PMID: 19066529.
BibTeX
@article{liu2008-19066529,
author = {Liu, L. and Duff, K.},
title = {{A technique for serial collection of cerebrospinal fluid from the cisterna magna in mouse}},
journal = {Journal of visualized experiments : JoVE},
year = {2008},
doi = {10.3791/960},
pmid = {19066529},
url = {https://doi.org/10.3791/960}
}
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Key findings
- This extremely gentle sampling technique allows serial CSF samples to be obtained from the same mouse at 2-3 month intervals which greatly minimizes the confounding effect of between-mouse variability in Abeta or tau levels, making it possible to detect subtle alterations over time.
- In combination with Abeta and tau ELISA, this technique will be useful for studies designed to investigate the relationship between the levels of CSF Abeta42 and tau, and their metabolism in the brain in AD mouse models.
- Studies in Tg mice could provide important validation as to the potential of CSF Abeta or tau levels to be used as biological markers for monitoring disease progression, and to monitor the effect of therapeutic interventions.
- As the mice can be sacrificed and the brains can be examined for biochemical or histological changes, the mechanisms underlying the CSF changes can be better assessed.
- These data are likely to be informative for interpretation of human AD CSF changes.
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Study details
- Evidence strengthLow
How this study scored 40/100
Published in a peer-reviewed journal. Scored zero for study design, sample size, effect reported and randomised / blinded because the record does not state it. We have no data on journal's citation record, times cited, citations per year, impact for its field and year, cited by studies here, still being cited, senior author's track record, institution's track record and multi-centre collaboration, so those were left out: it earned 23 of the 58 points we could judge it on, which is 40 out of 100.
| Component | Points | Why |
|---|---|---|
| Journal tier | 7 / 10 | Tier 3 — peer-reviewed journal (Journal of visualized experiments : JoVE) |
| Journal's citation record | — / 4 | No journal record from OpenAlex — left out of this score |
| Study design | 0 / 12 | No study design recorded — scores zero, not guessed (not stated in the record — scored 0) |
| Strength of evidence | 1 / 5 | Rated “low” in our summary |
| Peer-review validation | 10 / 10 | Peer-reviewed. Published as an article in a named peer-reviewed journal. |
| How recent | 1 / 5 | 2008 — within 20 year(s) of the newest study here |
| Sample size | 0 / 4 | Participant count not stated — scores zero (not stated in the record — scored 0) |
| Effect reported | 0 / 3 | No effect size reported — scores zero (not stated in the record — scored 0) |
| Studied in people | 0 / 3 | Laboratory or animal study (title names “mouse”) — scores zero |
| Randomised / blinded | 0 / 2 | No randomisation or blinding stated — scores zero (not stated in the record — scored 0) |
| Times cited | — / 7 | No citation count (not found in OpenAlex) — left out of this score |
| Citations per year | — / 5 | No citation count or no year — left out of this score |
| Impact for its field and year | — / 6 | No field-weighted citation impact (OpenAlex has none yet) — left out of this score |
| Cited by studies here | — / 6 | Not yet checked against this library's citation graph — left out of this score |
| Still being cited | — / 4 | No yearly citation counts — left out of this score |
| Senior author's track record | — / 4 | Senior author's record not available — left out of this score |
| Institution's track record | — / 3 | No institution listed in OpenAlex — left out of this score |
| Multi-centre collaboration | — / 3 | Institutions not listed — left out of this score |
| Indexed in PubMed | 2 / 2 | Indexed in PubMed (PMID 19066529) |
| Free to read | 2 / 2 | Free to read |
| Points | 23 / 58 | Scored on 11 of 20 parts. We have no data for the rest, so they are left out: 23 of the 58 points we could judge it on is 40 out of 100. |
| Total | 40 / 100 | — |
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