Home › Research › TREM2 and immune genetics
Transcriptomic atlas and interaction networks of brain cells in mouse CNS demyelination and remyelination
Hou J, Zhou Y, Cai Z, Terekhova M, Swain A, Andhey PS, Guimaraes RM, Ulezko Antonova A et al.. Cell reports, 2023-03-21
Topic: Brain proteins, microglia and lab models
Demyelination is a hallmark of multiple sclerosis, leukoencephalopathies, cerebral vasculopathies, and several neurodegenerative diseases. The cuprizone mouse model is widely used to simulate demyelination and remyelination occurring in these diseases. Here, we present a high-resolution single-nucleus RNA sequencing (snRNA-seq) analysis of gene expression changes across all brain cells in this model.
Key findings
- The cuprizone mouse model is widely used to simulate demyelination and remyelination occurring in these diseases.
- Here, we present a high-resolution single-nucleus RNA sequencing (snRNA-seq) analysis of gene expression changes across all brain cells in this model.
- We define demyelination-associated oligodendrocytes (DOLs) and remyelination-associated MAFB hi microglia, as well as astrocytes and vascular cells with signatures of altered metabolism, oxidative stress, and interferon response.
- Furthermore, snRNA-seq provides insights into how brain cell types connect and interact, defining complex circuitries that impact demyelination and remyelination.
Why it matters
This mouse study used single-nucleus RNA sequencing to map gene expression changes during demyelination and remyelination, identifying specific cell types. It is about demyelination models and does not report any treatment, cure, or prevention for Alzheimer's disease.
New to this subject? Start with our plain-English guide: Genetics →
Study details
- Evidence strengthLow
How this study scored 49/100
Published in a peer-reviewed journal. Scored zero for study design, sample size, effect reported and randomised / blinded because the record does not state it.
| Component | Points | Why |
|---|---|---|
| Journal tier | 7 / 10 | Tier 3 — peer-reviewed journal (Cell reports) |
| Journal's citation record | 3 / 4 | Cell Reports: journal h-index 321 (OpenAlex, 2026-10-29) |
| Study design | 0 / 12 | No study design recorded — scores zero, not guessed (not stated in the record — scored 0) |
| Strength of evidence | 1 / 5 | Rated “low” in our summary |
| Peer-review validation | 10 / 10 | Peer-reviewed. Published as an article in a named peer-reviewed journal. |
| How recent | 3 / 5 | 2023 — within 6 year(s) of the newest study here |
| Sample size | 0 / 4 | Participant count not stated — scores zero (not stated in the record — scored 0) |
| Effect reported | 0 / 3 | No effect size reported — scores zero (not stated in the record — scored 0) |
| Studied in people | 0 / 3 | Laboratory or animal study (title names “mouse”) — scores zero |
| Randomised / blinded | 0 / 2 | No randomisation or blinding stated — scores zero (not stated in the record — scored 0) |
| Times cited | 4 / 7 | Cited 85 times (OpenAlex, 2026-10-10) |
| Citations per year | 3 / 5 | 17.0 citations a year over 5 year(s) since 2023 |
| Impact for its field and year | 4 / 6 | Cited 14.2× the average for papers of its field and year (OpenAlex FWCI) |
| Cited by studies here | 0 / 6 | No other study in this library cites it |
| Still being cited | 3 / 4 | Cited 78 times in 2024–2026 (2026 still in progress; OpenAlex) |
| Senior author's track record | 4 / 4 | Last-listed author Marco Colonna: h-index 172 (OpenAlex, whole career) |
| Institution's track record | 2 / 3 | Washington University in St. Louis: institution h-index 1,962 (OpenAlex) |
| Multi-centre collaboration | 1 / 3 | OpenAlex lists 2 institution(s) in 2 countries |
| Indexed in PubMed | 2 / 2 | Indexed in PubMed (PMID 36952346) |
| Free to read | 2 / 2 | Free to read |
| Total | 49 / 100 | — |
What each of these 20 components measures, and what this score is not →
Read the original
Full text: free to read — OpenAlex lists this copy as free to read: the link above (checked 6 Feb 1020).
Cite this study APA, Vancouver, BibTeX · download .bib or .ris
Built from the record alzhub holds for this paper, not looked up. Volume, issue and page numbers are not held in the record. The record's author list ends in “et al.”, so not every author is named. Check the citation against the original before you use it.
APA 7th
Hou, J., Zhou, Y., Cai, Z., Terekhova, M., Swain, A., Andhey, P. S., et al. (2023). Transcriptomic atlas and interaction networks of brain cells in mouse CNS demyelination and remyelination. Cell reports. https://doi.org/10.1016/j.celrep.2023.112293
Vancouver
Hou J, Zhou Y, Cai Z, Terekhova M, Swain A, Andhey PS, et al. Transcriptomic atlas and interaction networks of brain cells in mouse CNS demyelination and remyelination. Cell reports. 2023. doi:10.1016/j.celrep.2023.112293. PMID: 36952346.
BibTeX
@article{hou2023-36952346,
author = {Hou, J. and Zhou, Y. and Cai, Z. and Terekhova, M. and Swain, A. and Andhey, P. S. and Guimaraes, R. M. and Ulezko Antonova, A. and others},
title = {{Transcriptomic atlas and interaction networks of brain cells in mouse CNS demyelination and remyelination}},
journal = {Cell reports},
year = {2023},
doi = {10.1016/j.celrep.2023.112293},
pmid = {36952346},
url = {https://doi.org/10.1016/j.celrep.2023.112293}
}
Studies this one cites
Referenced by this study and also in the alzhub library, so you can read them here.
Related studies in TREM2 and immune genetics
Also tagged “mice”
1,150 studies in the library carry the topic tag “mice”; these are this study's neighbours in a fixed, alphabetical-by-hash tour of them, not the closest in content. All 1,150 tagged studies.