High-throughput profiling of the mitochondrial proteome using affinity fractionation and automation
Mary F. Lopez, Bruce S. Kristal, Elena Chernokalskaya, Alexander V. Lazarev, Alexander I. Shestopalov, Alla Bogdanova, Myra H. Robinson. Electrophoresis, 2000-10-01
Topic: Disease mechanisms in lab and animal studies
Recent studies have demonstrated the need for complementing cellular genomic information with specific information on expressed proteins, or proteomics, since the correlation between the two is poor. Typically, proteomic information is gathered by analyzing samples on two-dimensional gels with the subsequent identification of specific proteins of interest by using trypsin digestion and mass spectrometry in a process termed peptide mass fingerprinting. These procedures have, as a rule, been labor-intensive and manual, and therefore of low throughput. The development of automated proteomic technology for processing large numbers of samples simultaneously has made the concept of profiling entire proteomes feasible at last. In this study, we report the initiation of the (eventual) complete profile of the rat mitochondrial proteome by using high-throughput automated equipment in combination with a novel fractionation technique using minispin affinity columns. Using these technologies, approximately one hundred proteins could be identified in several days. In addition, separate profiles of calcium binding proteins, glycoproteins, and hydrophobic or membrane proteins could be generated. Because mitochondrial dysfunction has been implicated in numerous diseases, such as cancer, Alzheimer's disease and diabetes, it is probable that the identification of the majority of mitochondrial proteins will be a beneficial tool for developing drug and diagnostic targets for associated diseases.
Key findings
- Recent studies have demonstrated the need for complementing cellular genomic information with specific information on expressed proteins, or proteomics, since the correlation between the two is poor.
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Study details
- Evidence strengthLow
How this study scored 36/100
Published in a peer-reviewed journal. Scored zero for study design, sample size, effect reported, studied in people and randomised / blinded because the record does not state it. We have no data on journal's citation record, times cited, citations per year, impact for its field and year, cited by studies here, still being cited, senior author's track record, institution's track record and multi-centre collaboration, so those were left out: it earned 21 of the 58 points we could judge it on, which is 36 out of 100.
| Component | Points | Why |
|---|---|---|
| Journal tier | 7 / 10 | Tier 3 — peer-reviewed journal (Electrophoresis) |
| Journal's citation record | — / 4 | No journal record from OpenAlex — left out of this score |
| Study design | 0 / 12 | No study design recorded — scores zero, not guessed (not stated in the record — scored 0) |
| Strength of evidence | 1 / 5 | Rated “low” in our summary |
| Peer-review validation | 10 / 10 | Peer-reviewed. Published as an article in a named peer-reviewed journal. |
| How recent | 1 / 5 | 2000 — older than 20 years, still counted |
| Sample size | 0 / 4 | Participant count not stated — scores zero (not stated in the record — scored 0) |
| Effect reported | 0 / 3 | No effect size reported — scores zero (not stated in the record — scored 0) |
| Studied in people | 0 / 3 | Not stated whether this was studied in people — scores zero (not stated in the record — scored 0) |
| Randomised / blinded | 0 / 2 | No randomisation or blinding stated — scores zero (not stated in the record — scored 0) |
| Times cited | — / 7 | No citation count (not found in OpenAlex) — left out of this score |
| Citations per year | — / 5 | No citation count or no year — left out of this score |
| Impact for its field and year | — / 6 | No field-weighted citation impact (OpenAlex has none yet) — left out of this score |
| Cited by studies here | — / 6 | Not yet checked against this library's citation graph — left out of this score |
| Still being cited | — / 4 | No yearly citation counts — left out of this score |
| Senior author's track record | — / 4 | Senior author's record not available — left out of this score |
| Institution's track record | — / 3 | No institution listed in OpenAlex — left out of this score |
| Multi-centre collaboration | — / 3 | Institutions not listed — left out of this score |
| Indexed in PubMed | 2 / 2 | Indexed in PubMed (PMID 11079563) |
| Free to read | 0 / 2 | Behind a paywall — scores zero |
| Points | 21 / 58 | Scored on 11 of 20 parts. We have no data for the rest, so they are left out: 21 of the 58 points we could judge it on is 36 out of 100. |
| Total | 36 / 100 | — |
What each of these 20 components measures, and what this score is not →
Read the original
- DOI: 10.1002/1522-2683(20001001)21:16<3427::aid-elps3427>3.0.co;2-l
- PubMed: 11079563
- Original source →
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Cite this study APA, Vancouver, BibTeX · download .bib or .ris
Built from the record alzhub holds for this paper, not looked up. Volume, issue and page numbers are not held in the record. The record names 7 authors; APA and Vancouver show the first six, BibTeX and the downloads carry all of them. Check the citation against the original before you use it.
APA 7th
Lopez, M. F., Kristal, B. S., Chernokalskaya, E., Lazarev, A. V., Shestopalov, A. I., Bogdanova, A., et al. (2000). High-throughput profiling of the mitochondrial proteome using affinity fractionation and automation. Electrophoresis. https://doi.org/10.1002/1522-2683(20001001)21:16<3427::aid-elps3427>3.0.co;2-l
Vancouver
Lopez MF, Kristal BS, Chernokalskaya E, Lazarev AV, Shestopalov AI, Bogdanova A, et al. High-throughput profiling of the mitochondrial proteome using affinity fractionation and automation. Electrophoresis. 2000. doi:10.1002/1522-2683(20001001)21:16<3427::aid-elps3427>3.0.co;2-l. PMID: 11079563.
BibTeX
@article{lopez2000-11079563,
author = {Lopez, Mary F. and Kristal, Bruce S. and Chernokalskaya, Elena and Lazarev, Alexander V. and Shestopalov, Alexander I. and Bogdanova, Alla and Robinson, Myra H.},
title = {{High-throughput profiling of the mitochondrial proteome using affinity fractionation and automation}},
journal = {Electrophoresis},
year = {2000},
doi = {10.1002/1522-2683(20001001)21:16<3427::aid-elps3427>3.0.co;2-l},
pmid = {11079563},
url = {https://doi.org/10.1002/1522-2683(20001001)21:16<3427::aid-elps3427>3.0.co;2-l}
}