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Amyloid β-peptide is produced by cultured cells during normal metabolism
Christian Haass, Michael G. Schlossmacher, Albert Y. Hung, Carmen Vigo‐Pelfrey, Angela Mellon, Beth L. Ostaszewski, Ivan M. Lieberburg, Edward H. Koo, Dale Schenk, David B. Teplow, Dennis J. Selkoe. Nature, 1992-09-01
Topic: Brain proteins, microglia and lab models
We do not hold a plain-English summary of this study yet. Use the links to the original below for its abstract and findings.
Key findings
- Unexpected identification of the 4K (M(r) 4,000) A beta and a truncated form of A beta (approximately 3K) in media from cultures of primary cells and untransfected and beta-APP-transfected cell lines grown under normal conditions.
- These peptides were immunoprecipitated readily from culture medium by A beta-specific antibodies and their identities confirmed by sequencing.
- A beta is produced in soluble form in vitro and in vivo during normal cellular metabolism.
- These findings provide the basis for using simple cell culture systems to identify drugs that block the formation or release of A beta.
Why it matters
Reports that cultured cells produce amyloid β-peptide during normal metabolism.
How this study scored 58/100
Published in a top-tier journal. Scored zero for study design, sample size, effect reported and randomised / blinded because the record does not state it. We have no data on strength of evidence, so that was left out: it earned 55 of the 95 points we could judge it on, which is 58 out of 100.
| Component | Points | Why |
|---|---|---|
| Journal tier | 10 / 10 | Tier 1 — top-tier general journal (Nature) |
| Journal's citation record | 4 / 4 | Nature: journal h-index 1,841 (OpenAlex, 2026-10-29) |
| Study design | 0 / 12 | No study design recorded — scores zero, not guessed (not stated in the record — scored 0) |
| Strength of evidence | — / 5 | No evidence rating recorded — left out of this score |
| Peer-review validation | 10 / 10 | Peer-reviewed. Published as an article in a named peer-reviewed journal. |
| How recent | 1 / 5 | 1992 — older than 20 years, still counted |
| Sample size | 0 / 4 | Participant count not stated — scores zero (not stated in the record — scored 0) |
| Effect reported | 0 / 3 | No effect size reported — scores zero (not stated in the record — scored 0) |
| Studied in people | 0 / 3 | Laboratory or animal study (title names “cultured”) — scores zero |
| Randomised / blinded | 0 / 2 | No randomisation or blinding stated — scores zero (not stated in the record — scored 0) |
| Times cited | 7 / 7 | Cited 1,926 times (OpenAlex, 2026-10-10) |
| Citations per year | 5 / 5 | 53.5 citations a year over 36 year(s) since 1992 |
| Impact for its field and year | 6 / 6 | Cited 75.9× the average for papers of its field and year (OpenAlex FWCI) |
| Cited by studies here | 0 / 6 | No other study in this library cites it |
| Still being cited | 2 / 4 | Cited 49 times in 2024–2026 (2026 still in progress; OpenAlex) |
| Senior author's track record | 4 / 4 | Last-listed author Dennis J. Selkoe: h-index 174 (OpenAlex, whole career) |
| Institution's track record | 3 / 3 | Harvard University: institution h-index 3,235 (OpenAlex) |
| Multi-centre collaboration | 1 / 3 | OpenAlex lists 3 institution(s) in 2 countries |
| Indexed in PubMed | 2 / 2 | Indexed in PubMed (PMID 1383826) |
| Free to read | 0 / 2 | Behind a paywall — scores zero |
| Points | 55 / 95 | Scored on 19 of 20 parts. We have no data for the rest, so they are left out: 55 of the 95 points we could judge it on is 58 out of 100. |
| Total | 58 / 100 | — |
What each of these 20 components measures, and what this score is not →
Read the original
May need a subscription OpenAlex found no free copy of the full text (checked 6 Feb 1020), so the publisher may charge for it. The PubMed abstract is free.
Cite this study APA, Vancouver, BibTeX · download .bib or .ris
Built from the record alzhub holds for this paper, not looked up. Volume, issue and page numbers are not held in the record. The record names 11 authors; APA and Vancouver show the first six, BibTeX and the downloads carry all of them. Check the citation against the original before you use it.
APA 7th
Haass, C., Schlossmacher, M. G., Hung, A. Y., Vigo‐Pelfrey, C., Mellon, A., Ostaszewski, B. L., et al. (1992). Amyloid β-peptide is produced by cultured cells during normal metabolism. Nature. https://doi.org/10.1038/359322a0
Vancouver
Haass C, Schlossmacher MG, Hung AY, Vigo‐Pelfrey C, Mellon A, Ostaszewski BL, et al. Amyloid β-peptide is produced by cultured cells during normal metabolism. Nature. 1992. doi:10.1038/359322a0. PMID: 1383826.
BibTeX
@article{haass1992-1383826,
author = {Haass, Christian and Schlossmacher, Michael G. and Hung, Albert Y. and Vigo‐Pelfrey, Carmen and Mellon, Angela and Ostaszewski, Beth L. and Lieberburg, Ivan M. and Koo, Edward H. and Schenk, Dale and Teplow, David B. and Selkoe, Dennis J.},
title = {{Amyloid β-peptide is produced by cultured cells during normal metabolism}},
journal = {Nature},
year = {1992},
doi = {10.1038/359322a0},
pmid = {1383826},
url = {https://doi.org/10.1038/359322a0}
}
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